A trimeric subdomain of the simian immunodeficiency virus envelope glycoprotein - PubMed (original) (raw)
Comparative Study
. 1995 Nov 21;34(46):14955-62.
doi: 10.1021/bi00046a001.
Affiliations
- PMID: 7578108
- DOI: 10.1021/bi00046a001
Comparative Study
A trimeric subdomain of the simian immunodeficiency virus envelope glycoprotein
S C Blacklow et al. Biochemistry. 1995.
Abstract
Previous attempts to define the oligomeric state of the HIV and SIV envelope glycoproteins have yielded conflicting results. We have produced in Escherichia coli a recombinant model for the ectodomain of the SIV envelope protein gp41 and have identified a small, trimeric subdomain by proteolytic digestion of this gp41 fragment. The subdomain assembles from two peptide fragments, spanning residues 28-80 (N28-80) and residues 107-149 (C107-149) of SIV gp41. Each of these peptides contains a 4,3-hydrophobic repeat, the hallmark of coiled-coil sequences. Upon mixing, the peptides form a highly helical, trimeric complex [3(N+C)] that resists proteolysis and has a melting temperature (Tm) above 90 degrees C in physiological buffer. The N- and C-terminal fragments are antiparallel to each other in the complex, as judged by the observation that digestion of a variant recombinant protein truncated at the amino terminus yields a C-terminal fragment shortened at its carboxy terminus. The N28-80 peptide contains more positions within the heptad repeat than C107-149 that are predominantly hydrophobic, suggesting that N28-80 is buried in the interior of the complex. We propose that the complex consists of a parallel, trimeric coiled-coil of the N-terminal peptide, encircled by three C-terminal peptide helices arranged in an antiparallel fashion, and that this complex forms a core within the gp41 extracellular domain.
Similar articles
- Buried polar interactions and conformational stability in the simian immunodeficiency virus (SIV) gp41 core.
Ji H, Bracken C, Lu M. Ji H, et al. Biochemistry. 2000 Feb 1;39(4):676-85. doi: 10.1021/bi991893e. Biochemistry. 2000. PMID: 10651632 - Crystal structure of the simian immunodeficiency virus (SIV) gp41 core: conserved helical interactions underlie the broad inhibitory activity of gp41 peptides.
Malashkevich VN, Chan DC, Chutkowski CT, Kim PS. Malashkevich VN, et al. Proc Natl Acad Sci U S A. 1998 Aug 4;95(16):9134-9. doi: 10.1073/pnas.95.16.9134. Proc Natl Acad Sci U S A. 1998. PMID: 9689046 Free PMC article. - Thermodynamics of trimer-of-hairpins formation by the SIV gp41 envelope protein.
Jelesarov I, Lu M. Jelesarov I, et al. J Mol Biol. 2001 Mar 23;307(2):637-56. doi: 10.1006/jmbi.2001.4469. J Mol Biol. 2001. PMID: 11254387 - SIV neutralization epitopes.
Javaherian K, Langlois AJ, LaRosa GJ. Javaherian K, et al. Chem Immunol. 1993;56:78-90. Chem Immunol. 1993. PMID: 7680868 Review. No abstract available.
Cited by
- The recent outbreaks of human coronaviruses: A medicinal chemistry perspective.
Pillaiyar T, Wendt LL, Manickam M, Easwaran M. Pillaiyar T, et al. Med Res Rev. 2021 Jan;41(1):72-135. doi: 10.1002/med.21724. Epub 2020 Aug 27. Med Res Rev. 2021. PMID: 32852058 Free PMC article. Review. - Enhancing natural killer cell function with gp41-targeting bispecific antibodies to combat HIV infection.
Ramadoss NS, Zhao NQ, Richardson BA, Grant PM, Kim PS, Blish CA. Ramadoss NS, et al. AIDS. 2020 Jul 15;34(9):1313-1323. doi: 10.1097/QAD.0000000000002543. AIDS. 2020. PMID: 32287071 Free PMC article. - Biphasic exocytosis of herpesvirus from hippocampal neurons and mechanistic implication to membrane fusion.
Liu YT, Shivakoti S, Jia F, Tao CL, Zhang B, Xu F, Lau P, Bi GQ, Zhou ZH. Liu YT, et al. Cell Discov. 2020 Jan 14;6:2. doi: 10.1038/s41421-019-0134-6. eCollection 2020. Cell Discov. 2020. PMID: 31969988 Free PMC article. - Biochemical Basis for Increased Activity of Ebola Glycoprotein in the 2013-16 Epidemic.
Wang MK, Lim SY, Lee SM, Cunningham JM. Wang MK, et al. Cell Host Microbe. 2017 Mar 8;21(3):367-375. doi: 10.1016/j.chom.2017.02.002. Epub 2017 Feb 23. Cell Host Microbe. 2017. PMID: 28238624 Free PMC article. - MERS-CoV spike protein: Targets for vaccines and therapeutics.
Wang Q, Wong G, Lu G, Yan J, Gao GF. Wang Q, et al. Antiviral Res. 2016 Sep;133:165-77. doi: 10.1016/j.antiviral.2016.07.015. Epub 2016 Jul 26. Antiviral Res. 2016. PMID: 27468951 Free PMC article. Review.
Publication types
MeSH terms
Substances
LinkOut - more resources
Other Literature Sources