Study of the organization of the genomes of Escherichia coli, Brucella melitensis and Agrobacterium tumefaciens by insertion of a unique restriction site - PubMed (original) (raw)
Comparative Study
. 1995 Oct:141 ( Pt 10):2425-32.
doi: 10.1099/13500872-141-10-2425.
Affiliations
- PMID: 7582002
- DOI: 10.1099/13500872-141-10-2425
Free article
Comparative Study
Study of the organization of the genomes of Escherichia coli, Brucella melitensis and Agrobacterium tumefaciens by insertion of a unique restriction site
E Jumas-Bilak et al. Microbiology (Reading). 1995 Oct.
Free article
Abstract
Tn5Map, a Tn5 derivative containing the 18 bp I-SceI site, was delivered from a RP4-mobilizable, RK6-derived suicide vector to Escherichia coli HB101, Brucella melitensis and Agrobacterium tumefaciens C58, which all lack natural I-SceI sites in their genomes. Digestion of the DNA from Tn5Map-containing strains and analysis by pulsed-field gel electrophoresis (PFGE) revealed that these derivatives contained a single transposon insertion. These digests also gave direct and independent proof for the single circular chromosome of E. coli, and for the presence of two circular chromosomes in B. melitensis and of a circular and a linear chromosome in A. tumefaciens C58 (which also contains two large circular plasmids). This rapid and versatile technique is potentially applicable to the study of the genomic organization in all Gram-negative bacteria which support Tn5 transposition. Moreover, linearization of circular replicons could be the first step for a rapid method of physical mapping.
Similar articles
- Presence of one linear and one circular chromosome in the Agrobacterium tumefaciens C58 genome.
Allardet-Servent A, Michaux-Charachon S, Jumas-Bilak E, Karayan L, Ramuz M. Allardet-Servent A, et al. J Bacteriol. 1993 Dec;175(24):7869-74. doi: 10.1128/jb.175.24.7869-7874.1993. J Bacteriol. 1993. PMID: 8253676 Free PMC article. - Presence of two independent chromosomes in the Brucella melitensis 16M genome.
Michaux S, Paillisson J, Carles-Nurit MJ, Bourg G, Allardet-Servent A, Ramuz M. Michaux S, et al. J Bacteriol. 1993 Feb;175(3):701-5. doi: 10.1128/jb.175.3.701-705.1993. J Bacteriol. 1993. PMID: 8423146 Free PMC article. - Genome-wide sequence transposon insertion sites and analyze the essential genes of Brucella melitensis.
De Y, Dong C, Cao Y, Wang X, Yang X, Wang N, Zhang C, Wang Z, Lyu Y, Wu Q. De Y, et al. Microb Pathog. 2017 Nov;112:97-102. doi: 10.1016/j.micpath.2017.09.005. Epub 2017 Sep 6. Microb Pathog. 2017. PMID: 28888882 - Integration of genes into the chromosome of Agrobacterium tumefaciens C58.
Lee LY. Lee LY. Methods Mol Biol. 2006;343:55-66. doi: 10.1385/1-59745-130-4:55. Methods Mol Biol. 2006. PMID: 16988333 Review. - The genome of Brucella melitensis.
DelVecchio VG, Kapatral V, Elzer P, Patra G, Mujer CV. DelVecchio VG, et al. Vet Microbiol. 2002 Dec 20;90(1-4):587-92. doi: 10.1016/s0378-1135(02)00238-9. Vet Microbiol. 2002. PMID: 12414174 Review.
Cited by
- Reexamining the role of the accessory plasmid pAtC58 in the virulence of Agrobacterium tumefaciens strain C58.
Nair GR, Liu Z, Binns AN. Nair GR, et al. Plant Physiol. 2003 Nov;133(3):989-99. doi: 10.1104/pp.103.030262. Epub 2003 Oct 9. Plant Physiol. 2003. PMID: 14551325 Free PMC article. - Combined genetic and physical map of the complex genome of Agrobacterium tumefaciens.
Goodner BW, Markelz BP, Flanagan MC, Crowell CB Jr, Racette JL, Schilling BA, Halfon LM, Mellors JS, Grabowski G. Goodner BW, et al. J Bacteriol. 1999 Sep;181(17):5160-6. doi: 10.1128/JB.181.17.5160-5166.1999. J Bacteriol. 1999. PMID: 10464183 Free PMC article. - Essential components of the Ti plasmid trb system, a type IV macromolecular transporter.
Li PL, Hwang I, Miyagi H, True H, Farrand SK. Li PL, et al. J Bacteriol. 1999 Aug;181(16):5033-41. doi: 10.1128/JB.181.16.5033-5041.1999. J Bacteriol. 1999. PMID: 10438776 Free PMC article. - Unconventional genomic organization in the alpha subgroup of the Proteobacteria.
Jumas-Bilak E, Michaux-Charachon S, Bourg G, Ramuz M, Allardet-Servent A. Jumas-Bilak E, et al. J Bacteriol. 1998 May;180(10):2749-55. doi: 10.1128/JB.180.10.2749-2755.1998. J Bacteriol. 1998. PMID: 9573163 Free PMC article. - Epidemiological study of a food-borne outbreak of enterotoxigenic Escherichia coli O25:NM by pulsed-field gel electrophoresis and randomly amplified polymorphic DNA analysis.
Mitsuda T, Muto T, Yamada M, Kobayashi N, Toba M, Aihara Y, Ito A, Yokota S. Mitsuda T, et al. J Clin Microbiol. 1998 Mar;36(3):652-6. doi: 10.1128/JCM.36.3.652-656.1998. J Clin Microbiol. 1998. PMID: 9508290 Free PMC article.