Participation of the NG2 proteoglycan in rat aortic smooth muscle cell responses to platelet-derived growth factor - PubMed (original) (raw)
Participation of the NG2 proteoglycan in rat aortic smooth muscle cell responses to platelet-derived growth factor
K A Grako et al. Exp Cell Res. 1995 Nov.
Abstract
Through immunohistochemical studies we have identified the cell-surface proteoglycan, NG2, on blood vessels throughout the rat embryo. The particular cell type expressing this chondroitin sulfate proteoglycan, however, is dependent upon tissue location. Microvessels within the rat CNS express NG2 on endothelial cells, while in blood vessels outside the CNS, NG2 is found on smooth muscle cells. To analyze what role NG2 might play in these blood vessels, an enzymatic dissociation protocol was used to establish primary cultures of vascular smooth muscle cells from Postnatal Day 3 rat aorta. In this study we demonstrate the involvement of NG2 in the mitogenic and chemoattractant responses of smooth muscle cells to PDGF. In assays measuring either DNA synthesis or cell migration, treatment of smooth muscle cells with anti-NG2 immunoglobulins decreased their responses to PDGF-AA but had no effect upon their ability to react to PDGF-BB. These results support a role for NG2 in potentiating signaling through the alpha PDGF receptor in vascular smooth muscle cells. The presence of the proteoglycan on a large subpopulation of these cells could provide an enhanced response to the growth factor in times of active normal growth or in pathological conditions, such as arterial injury or atherosclerosis.
Similar articles
- PDGF (alpha)-receptor is unresponsive to PDGF-AA in aortic smooth muscle cells from the NG2 knockout mouse.
Grako KA, Ochiya T, Barritt D, Nishiyama A, Stallcup WB. Grako KA, et al. J Cell Sci. 1999 Mar;112 ( Pt 6):905-15. doi: 10.1242/jcs.112.6.905. J Cell Sci. 1999. PMID: 10036240 - Pregnancy-stimulated growth of vascular smooth muscle cells: importance of protein kinase C-dependent synergy between estrogen and platelet-derived growth factor.
Keyes LE, Moore LG, Walchak SJ, Dempsey EC. Keyes LE, et al. J Cell Physiol. 1996 Jan;166(1):22-32. doi: 10.1002/(SICI)1097-4652(199601)166:1<22::AID-JCP3>3.0.CO;2-I. J Cell Physiol. 1996. PMID: 8557771 - Smooth muscle cells isolated from the neointima after vascular injury exhibit altered responses to platelet-derived growth factor and other stimuli.
Majack RA, Grieshaber NA, Cook CL, Weiser MC, McFall RC, Grieshaber SS, Reidy MA, Reilly CF. Majack RA, et al. J Cell Physiol. 1996 Apr;167(1):106-12. doi: 10.1002/(SICI)1097-4652(199604)167:1<106::AID-JCP12>3.0.CO;2-9. J Cell Physiol. 1996. PMID: 8698827 - The NG2 chondroitin sulfate proteoglycan: a multifunctional proteoglycan associated with immature cells.
Levine JM, Nishiyama A. Levine JM, et al. Perspect Dev Neurobiol. 1996;3(4):245-59. Perspect Dev Neurobiol. 1996. PMID: 9117258 Review. - [PDGF system in vascular smooth muscle cell proliferation].
Matsui T. Matsui T. Nihon Rinsho. 1993 Jun;51(6):1656-62. Nihon Rinsho. 1993. PMID: 8320845 Review. Japanese.
Cited by
- Chondroitin Sulfate Proteoglycan 4 as a Marker for Aggressive Squamous Cell Carcinoma.
Chen K, Yong J, Zauner R, Wally V, Whitelock J, Sajinovic M, Kopecki Z, Liang K, Scott KF, Mellick AS. Chen K, et al. Cancers (Basel). 2022 Nov 13;14(22):5564. doi: 10.3390/cancers14225564. Cancers (Basel). 2022. PMID: 36428658 Free PMC article. Review. - Differential Modulators of NG2-Glia Differentiation into Neurons and Glia and Their Crosstalk.
Du X, Zhang Z, Zhou H, Zhou J. Du X, et al. Cell Mol Neurobiol. 2021 Jan;41(1):1-15. doi: 10.1007/s10571-020-00843-0. Epub 2020 Apr 13. Cell Mol Neurobiol. 2021. PMID: 32285247 Review. - NG2 Proteoglycan Enhances Brain Tumor Progression by Promoting Beta-1 Integrin Activation in both Cis and Trans Orientations.
Stallcup WB. Stallcup WB. Cancers (Basel). 2017 Mar 31;9(4):31. doi: 10.3390/cancers9040031. Cancers (Basel). 2017. PMID: 28362324 Free PMC article. Review. - Roles of NG2-glia in ischemic stroke.
Song FE, Huang JL, Lin SH, Wang S, Ma GF, Tong XP. Song FE, et al. CNS Neurosci Ther. 2017 Jul;23(7):547-553. doi: 10.1111/cns.12690. Epub 2017 Mar 19. CNS Neurosci Ther. 2017. PMID: 28317272 Free PMC article. Review. - The Role of NG2 Proteoglycan in Glioma.
Yadavilli S, Hwang EI, Packer RJ, Nazarian J. Yadavilli S, et al. Transl Oncol. 2016 Feb;9(1):57-63. doi: 10.1016/j.tranon.2015.12.005. Transl Oncol. 2016. PMID: 26947882 Free PMC article. Review.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources