Sterol regulation of fatty acid synthase promoter. Coordinate feedback regulation of two major lipid pathways - PubMed (original) (raw)
. 1995 Oct 27;270(43):25578-83.
doi: 10.1074/jbc.270.43.25578.
Affiliations
- PMID: 7592729
- DOI: 10.1074/jbc.270.43.25578
Free article
Sterol regulation of fatty acid synthase promoter. Coordinate feedback regulation of two major lipid pathways
M K Bennett et al. J Biol Chem. 1995.
Free article
Abstract
The gene encoding fatty acid synthase, the essential multi-functional enzyme of fatty acid biosynthesis, is shown to be regulated by cellular sterol levels similar to genes that encode important proteins of cholesterol metabolism. We show that expression of the endogenous FAS gene is repressed when regulatory sterols are included in the culture medium of HepG2 cells and that the FAS promoter is subject to similar regulation when fused to the luciferase reporter gene. Mutational studies demonstrate that sterol regulation is mediated by binding sites for the sterol regulatory element-binding protein (SREBP) and transcription factor Sp1, making it mechanistically similar to sterol regulation of the low density lipoprotein receptor gene. It is also demonstrated that SREBP and Sp1 synergistically activate the FAS promoter in Drosophila tissue culture cells, which lack endogenous Sp1. These experiments provide key molecular evidence that directly links the metabolism of fatty acids and cholesterol together.
Similar articles
- Cooperation by sterol regulatory element-binding protein and Sp1 in sterol regulation of low density lipoprotein receptor gene.
Sanchez HB, Yieh L, Osborne TF. Sanchez HB, et al. J Biol Chem. 1995 Jan 20;270(3):1161-9. doi: 10.1074/jbc.270.3.1161. J Biol Chem. 1995. PMID: 7836375 - Co-stimulation of promoter for low density lipoprotein receptor gene by sterol regulatory element-binding protein and Sp1 is specifically disrupted by the yin yang 1 protein.
Bennett MK, Ngo TT, Athanikar JN, Rosenfeld JM, Osborne TF. Bennett MK, et al. J Biol Chem. 1999 May 7;274(19):13025-32. doi: 10.1074/jbc.274.19.13025. J Biol Chem. 1999. PMID: 10224053 - Sterol regulatory element-binding proteins: activators of cholesterol and fatty acid biosynthesis.
Horton JD, Shimomura I. Horton JD, et al. Curr Opin Lipidol. 1999 Apr;10(2):143-50. doi: 10.1097/00041433-199904000-00008. Curr Opin Lipidol. 1999. PMID: 10327282 Review. - Sterol regulatory element binding proteins (SREBPs): controllers of lipid synthesis and cellular uptake.
Brown MS, Goldstein JL. Brown MS, et al. Nutr Rev. 1998 Feb;56(2 Pt 2):S1-3; discussion S54-75. doi: 10.1111/j.1753-4887.1998.tb01680.x. Nutr Rev. 1998. PMID: 9564170 Review.
Cited by
- Dimethyloxalylglycine Suppresses SREBP1c and Lipogenic Gene Expressions in Hepatocytes Independently of HIF1A.
Kwon YS, Cho YE, Kim Y, Koh M, Hwang S. Kwon YS, et al. Curr Issues Mol Biol. 2024 Mar 13;46(3):2386-2397. doi: 10.3390/cimb46030151. Curr Issues Mol Biol. 2024. PMID: 38534767 Free PMC article. - Micro-Executor of Natural Products in Metabolic Diseases.
Liu J, Chen H, Li X, Song C, Wang L, Wang D. Liu J, et al. Molecules. 2023 Aug 23;28(17):6202. doi: 10.3390/molecules28176202. Molecules. 2023. PMID: 37687031 Free PMC article. Review. - Elucidation of sterol biosynthesis pathway and its co-regulation with fatty acid biosynthesis in the oleaginous marine protist Schizochytrium sp.
Bi Y, Guo P, Liu L, Chen L, Zhang W. Bi Y, et al. Front Bioeng Biotechnol. 2023 Apr 27;11:1188461. doi: 10.3389/fbioe.2023.1188461. eCollection 2023. Front Bioeng Biotechnol. 2023. PMID: 37180050 Free PMC article. - Fatty Acid Synthase: Structure, Function, and Regulation.
Günenc AN, Graf B, Stark H, Chari A. Günenc AN, et al. Subcell Biochem. 2022;99:1-33. doi: 10.1007/978-3-031-00793-4_1. Subcell Biochem. 2022. PMID: 36151372 Review. - Lipid Metabolism in Glioblastoma: From De Novo Synthesis to Storage.
Kou Y, Geng F, Guo D. Kou Y, et al. Biomedicines. 2022 Aug 11;10(8):1943. doi: 10.3390/biomedicines10081943. Biomedicines. 2022. PMID: 36009491 Free PMC article. Review.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Research Materials
Miscellaneous