Differential utilization of IFN-gamma-responsive elements in two maturationally distinct macrophage cell lines - PubMed (original) (raw)
. 1995 Nov 15;155(10):4933-8.
Affiliations
- PMID: 7594498
Differential utilization of IFN-gamma-responsive elements in two maturationally distinct macrophage cell lines
M A McDowell et al. J Immunol. 1995.
Abstract
We have characterized the transcriptional response to IFN-gamma in two maturationally distinct macrophage populations: the mature RAW 264.7 cell line, phenotypically identical to thioglycollate-elicited peritoneal macrophages, and the less mature WEHI-3 cell line. We first investigated the use of two IFN-gamma-responsive regulatory elements, the interferon-stimulated response element (ISRE) and the gamma-activated sequence (GAS), in these cells. Transient transfection assays revealed that synthetic promoter constructs containing either the ISRE or GAS regulatory motif fused to a luciferase reporter gene were transcriptionally inactive in the WEHI-3 cell line. We then analyzed the expression in the two cell lines of a panel of known IFN-gamma-responsive genes that are transcriptionally controlled by different regulatory elements. RT-PCR analysis revealed that both cell lines responded to IFN-gamma treatment by up-regulating genes that are transcriptionally controlled by kappa B or W box DNA binding motifs. However, genes regulated by ISRE or GAS elements were induced by IFN-gamma only in the RAW 264.7 cell line. Kinetic analysis of the transcriptional activity of synthetic promoter constructs in the RAW 264.7 cell line showed rapid IFN-gamma induction through both the ISRE and GAS motifs, indicating that both elements are utilized early after IFN-gamma stimulation in mature macrophages. These results suggest that cis-acting DNA response element utilization, and the subsequent profiles of IFN-gamma-induced gene expression, differ in macrophages at different stages of maturation.
Similar articles
- Promoter analysis of an interferon-inducible gene associated with macrophage activation.
Nicolet CM, Paulnock DM. Nicolet CM, et al. J Immunol. 1994 Jan 1;152(1):153-62. J Immunol. 1994. PMID: 8254188 - Interferon-gamma regulation of the human mimecan promoter.
Tasheva ES, Conrad GW. Tasheva ES, et al. Mol Vis. 2003 Jun 30;9:277-87. Mol Vis. 2003. PMID: 12835654 - 1alpha-Hydroxylase transactivation by gamma-interferon in murine macrophages requires enhanced C/EBPbeta expression and activation.
Esteban L, Vidal M, Dusso A. Esteban L, et al. J Steroid Biochem Mol Biol. 2004 May;89-90(1-5):131-7. doi: 10.1016/j.jsbmb.2004.03.092. J Steroid Biochem Mol Biol. 2004. PMID: 15225760
Cited by
- Toxoplasma gondii actively remodels the microtubule network in host cells.
Walker ME, Hjort EE, Smith SS, Tripathi A, Hornick JE, Hinchcliffe EH, Archer W, Hager KM. Walker ME, et al. Microbes Infect. 2008 Nov-Dec;10(14-15):1440-9. doi: 10.1016/j.micinf.2008.08.014. Epub 2008 Oct 17. Microbes Infect. 2008. PMID: 18983931 Free PMC article. - Interferon (IFN) consensus sequence-binding protein, a transcription factor of the IFN regulatory factor family, regulates immune responses in vivo through control of interleukin 12 expression.
Giese NA, Gabriele L, Doherty TM, Klinman DM, Tadesse-Heath L, Contursi C, Epstein SL, Morse HC 3rd. Giese NA, et al. J Exp Med. 1997 Nov 3;186(9):1535-46. doi: 10.1084/jem.186.9.1535. J Exp Med. 1997. PMID: 9348311 Free PMC article. - Multiple control elements mediate activation of the murine and human interleukin 12 p40 promoters: evidence of functional synergy between C/EBP and Rel proteins.
Plevy SE, Gemberling JH, Hsu S, Dorner AJ, Smale ST. Plevy SE, et al. Mol Cell Biol. 1997 Aug;17(8):4572-88. doi: 10.1128/MCB.17.8.4572. Mol Cell Biol. 1997. PMID: 9234715 Free PMC article. - The altered tumoricidal capacity of macrophages isolated from tumor-bearing mice is related to reduce expression of the inducible nitric oxide synthase gene.
Dinapoli MR, Calderon CL, Lopez DM. Dinapoli MR, et al. J Exp Med. 1996 Apr 1;183(4):1323-9. doi: 10.1084/jem.183.4.1323. J Exp Med. 1996. PMID: 8666890 Free PMC article.