Molecular cloning of the transcription factor TFIIB homolog from Sulfolobus shibatae - PubMed (original) (raw)
Comparative Study
Molecular cloning of the transcription factor TFIIB homolog from Sulfolobus shibatae
S A Qureshi et al. Proc Natl Acad Sci U S A. 1995.
Abstract
The Archaea (archaebacteria) constitute a group of prokaryotes that are phylogenetically distinct from Eucarya (eukaryotes) and Bacteria (eubacteria). Although Archaea possess only one RNA polymerase, evidence suggests that their transcriptional apparatus is similar to that of Eucarya. For example, Archaea contain a homolog of the TATA-binding protein which interacts with the TATA-box like A-box sequence upstream of many archaeal genes. Here, we report the cloning of a Sulfolobus shibatae gene that encodes a protein (transcription factor TFB) with striking homology to the eukaryotic basal transcription factor TFIIB. We show by primer extension analysis that transcription of the S. shibatae TFB gene initiates 27 bp downstream from a consensus A-box element. Significantly, S. shibatae TFB contains an N-terminal putative metal-binding region and two imperfect direct repeats--structural features that are well conserved in eukaryotic TFIIBs. This suggests that TFB may perform analogous functions in Archaea and Eucarya. Consistent with this, we demonstrate that S. shibatae TFB promotes the binding of S. shibatae TBP to the A-box element of the Sulfolobus 16S/23S rRNA gene. Finally, we show that S. shibatae TFB is significantly more related to TFB of the archaeon Pyrococcus woesei than it is to eukaryotic TFIIBs. These data suggest that TFB arose in the common archaeal/eukaryotic ancestor and that the lineages leading to P. woesei and S. shibatae separated after the divergence of the archaeal and eukaryotic lines of descent.
Similar articles
- Cloning and functional analysis of the TATA binding protein from Sulfolobus shibatae.
Qureshi SA, Baumann P, Rowlands T, Khoo B, Jackson SP. Qureshi SA, et al. Nucleic Acids Res. 1995 May 25;23(10):1775-81. doi: 10.1093/nar/23.10.1775. Nucleic Acids Res. 1995. PMID: 7784182 Free PMC article. - Factor requirements for transcription in the Archaeon Sulfolobus shibatae.
Qureshi SA, Bell SD, Jackson SP. Qureshi SA, et al. EMBO J. 1997 May 15;16(10):2927-36. doi: 10.1093/emboj/16.10.2927. EMBO J. 1997. PMID: 9184236 Free PMC article. - Sequence-specific DNA binding by the S. shibatae TFIIB homolog, TFB, and its effect on promoter strength.
Qureshi SA, Jackson SP. Qureshi SA, et al. Mol Cell. 1998 Feb;1(3):389-400. doi: 10.1016/s1097-2765(00)80039-8. Mol Cell. 1998. PMID: 9660923 - Archaeal chromatin and transcription.
Reeve JN. Reeve JN. Mol Microbiol. 2003 May;48(3):587-98. doi: 10.1046/j.1365-2958.2003.03439.x. Mol Microbiol. 2003. PMID: 12694606 Review. - Archaeal transcription factors and their role in transcription initiation.
Thomm M. Thomm M. FEMS Microbiol Rev. 1996 May;18(2-3):159-71. doi: 10.1111/j.1574-6976.1996.tb00234.x. FEMS Microbiol Rev. 1996. PMID: 8639326 Review.
Cited by
- The cutting edge of archaeal transcription.
Fouqueau T, Blombach F, Cackett G, Carty AE, Matelska DM, Ofer S, Pilotto S, Phung DK, Werner F. Fouqueau T, et al. Emerg Top Life Sci. 2018 Dec 14;2(4):517-533. doi: 10.1042/ETLS20180014. Emerg Top Life Sci. 2018. PMID: 33525828 Free PMC article. - A whole-genome approach to identifying protein binding sites: promoters in Methanocaldococcus (Methanococcus) jannaschii.
Li E, Reich CI, Olsen GJ. Li E, et al. Nucleic Acids Res. 2008 Dec;36(22):6948-58. doi: 10.1093/nar/gkm499. Epub 2008 Nov 3. Nucleic Acids Res. 2008. PMID: 18981048 Free PMC article. - Archaeal transcription: function of an alternative transcription factor B from Pyrococcus furiosus.
Micorescu M, Grünberg S, Franke A, Cramer P, Thomm M, Bartlett M. Micorescu M, et al. J Bacteriol. 2008 Jan;190(1):157-67. doi: 10.1128/JB.01498-07. Epub 2007 Oct 26. J Bacteriol. 2008. PMID: 17965161 Free PMC article. - TFIIB and the regulation of transcription by RNA polymerase II.
Deng W, Roberts SG. Deng W, et al. Chromosoma. 2007 Oct;116(5):417-29. doi: 10.1007/s00412-007-0113-9. Epub 2007 Jun 26. Chromosoma. 2007. PMID: 17593382 Review. - Production of recombinant and tagged proteins in the hyperthermophilic archaeon Sulfolobus solfataricus.
Albers SV, Jonuscheit M, Dinkelaker S, Urich T, Kletzin A, Tampé R, Driessen AJ, Schleper C. Albers SV, et al. Appl Environ Microbiol. 2006 Jan;72(1):102-11. doi: 10.1128/AEM.72.1.102-111.2006. Appl Environ Microbiol. 2006. PMID: 16391031 Free PMC article.
References
- Proc Natl Acad Sci U S A. 1990 Jun;87(12):4576-9 - PubMed
- J Biol Chem. 1994 Dec 2;269(48):30569-73 - PubMed
- J Mol Biol. 1990 Oct 5;215(3):403-10 - PubMed
- Nucleic Acids Res. 1994 Nov 11;22(22):4673-80 - PubMed
- Genes Dev. 1994 Dec 1;8(23):2879-90 - PubMed
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases