The p150 and p60 subunits of chromatin assembly factor I: a molecular link between newly synthesized histones and DNA replication - PubMed (original) (raw)

The p150 and p60 subunits of chromatin assembly factor I: a molecular link between newly synthesized histones and DNA replication

P D Kaufman et al. Cell. 1995.

Free article

Abstract

Chromatin assembly factor I (CAF-I) from human cell nuclei is a three-subunit protein complex that assembles histone octamers onto replicating DNA in a cell-free system. Sequences of cDNAs encoding the two largest CAF-I subunits reveal that the p150 protein contains large clusters of charged residues, whereas p60 contains WD repeats. p150 and p60 directly interact and are both required for DNA replication-dependent assembly of nucleosomes. Deletion of the p60-binding domain from the p150 protein prevents chromatin assembly. p150 and p60 form complexes with newly synthesized histones H3 and acetylated H4 in human cell extracts, suggesting that such complexes are intermediates between histone synthesis and assembly onto replicating DNA.

PubMed Disclaimer

Similar articles

• Nucleosome assembly by a complex of CAF-1 and acetylated histones H3/H4.
  Verreault A, Kaufman PD, Kobayashi R, Stillman B. Verreault A, et al. Cell. 1996 Oct 4;87(1):95-104. doi: 10.1016/s0092-8674(00)81326-4. Cell. 1996. PMID: 8858152
• Ultraviolet radiation sensitivity and reduction of telomeric silencing in Saccharomyces cerevisiae cells lacking chromatin assembly factor-I.
  Kaufman PD, Kobayashi R, Stillman B. Kaufman PD, et al. Genes Dev. 1997 Feb 1;11(3):345-57. doi: 10.1101/gad.11.3.345. Genes Dev. 1997. PMID: 9030687
• The p55 subunit of Drosophila chromatin assembly factor 1 is homologous to a histone deacetylase-associated protein.
  Tyler JK, Bulger M, Kamakaka RT, Kobayashi R, Kadonaga JT. Tyler JK, et al. Mol Cell Biol. 1996 Nov;16(11):6149-59. doi: 10.1128/MCB.16.11.6149. Mol Cell Biol. 1996. PMID: 8887645 Free PMC article.
• Chromatin assembly during DNA replication in somatic cells.
  Krude T. Krude T. Eur J Biochem. 1999 Jul;263(1):1-5. doi: 10.1046/j.1432-1327.1999.00508.x. Eur J Biochem. 1999. PMID: 10429179 Review.
• The role of the chromatin assembly complex (CAF-1) and its p60 subunit (CHAF1b) in homeostasis and disease.
  Volk A, Crispino JD. Volk A, et al. Biochim Biophys Acta. 2015 Aug;1849(8):979-86. doi: 10.1016/j.bbagrm.2015.05.009. Epub 2015 Jun 9. Biochim Biophys Acta. 2015. PMID: 26066981 Free PMC article. Review.

Cited by

• Acute multi-level response to defective de novo chromatin assembly in S-phase.
  Dreyer J, Ricci G, van den Berg J, Bhardwaj V, Funk J, Armstrong C, van Batenburg V, Sine C, VanInsberghe MA, Marsman R, Mandemaker IK, di Sanzo S, Costantini J, Manzo SG, Biran A, Burny C, Völker-Albert M, Groth A, Spencer SL, van Oudenaarden A, Mattiroli F. Dreyer J, et al. bioRxiv [Preprint]. 2024 Mar 27:2024.03.22.586291. doi: 10.1101/2024.03.22.586291. bioRxiv. 2024. PMID: 38585916 Free PMC article. Preprint.
• Disordered regions and folded modules in CAF-1 promote histone deposition in Schizosaccharomyces pombe.
  Ouasti F, Audin M, Fréon K, Quivy JP, Tachekort M, Cesard E, Thureau A, Ropars V, Fernández Varela P, Moal G, Soumana-Amadou I, Uryga A, Legrand P, Andreani J, Guerois R, Almouzni G, Lambert S, Ochsenbein F. Ouasti F, et al. Elife. 2024 Feb 20;12:RP91461. doi: 10.7554/eLife.91461. Elife. 2024. PMID: 38376141 Free PMC article.
• Predicted deleterious variants in the human genome relevant to gene therapy with adeno-associated virus vectors.
  Rostami MR, Leopold PL, Vasquez JM, de Mulder Rougvie M, Al Shakaki A, Hssain AA, Robay A, Hackett NR, Mezey JG, Crystal RG. Rostami MR, et al. Mol Ther Methods Clin Dev. 2023 Oct 13;31:101136. doi: 10.1016/j.omtm.2023.101136. eCollection 2023 Dec 14. Mol Ther Methods Clin Dev. 2023. PMID: 38089635 Free PMC article.
• Circular RNA in Non-small Cell Lung Carcinoma: Identification of Targets and New Treatment Modalities.
  Weidle UH, Birzele F. Weidle UH, et al. Cancer Genomics Proteomics. 2023 Dec;20(6suppl):646-668. doi: 10.21873/cgp.20413. Cancer Genomics Proteomics. 2023. PMID: 38035705 Free PMC article. Review.
• Chromatin assembly factor-1 preserves genome stability in _ctf4_Δ cells by promoting sister chromatid cohesion.
  Ghaddar N, Luciano P, Géli V, Corda Y. Ghaddar N, et al. Cell Stress. 2023 Aug 14;7(9):69-89. doi: 10.15698/cst2023.09.289. eCollection 2023 Sep 11. Cell Stress. 2023. PMID: 37662646 Free PMC article.

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Elsevier Science

• Other Literature Sources

  • The Lens - Patent Citations

• Molecular Biology Databases

  • BioCyc
  • Gene Ontology
  • GlyGen glycoinformatics resource