Capturing genes encoding membrane and secreted proteins important for mouse development - PubMed (original) (raw)

Capturing genes encoding membrane and secreted proteins important for mouse development

W C Skarnes et al. Proc Natl Acad Sci U S A. 1995.

Abstract

A strategy based on the gene trap was developed to prescreen mouse embryonic stem cells for insertional mutations in genes encoding secreted and membrane-spanning proteins. The "secretory trap" relies on capturing the N-terminal signal sequence of an endogenous gene to generate an active beta-galactosidase fusion protein. Insertions were found in a cadherin gene, an unc6-related laminin (netrin) gene, the sek receptor tyrosine kinase gene, and genes encoding two receptor-linked protein-tyrosine phosphatases, LAR and PTP kappa. Analysis of homozygous mice carrying insertions in LAR and PTP kappa showed that both genes were effectively disrupted, but neither was essential for normal embryonic development.

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References

    1. Genes Dev. 1994 May 1;8(9):1087-105 - PubMed
    1. Cell. 1994 Aug 12;78(3):425-35 - PubMed
    1. Proc Natl Acad Sci U S A. 1987 Mar;84(6):1649-53 - PubMed
    1. J Exp Med. 1988 Nov 1;168(5):1523-30 - PubMed
    1. Proc Natl Acad Sci U S A. 1988 Dec;85(23):8998-9002 - PubMed

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