Characterization of a new 60 kDa apical protein of Plasmodium falciparum merozoite expressed in late schizogony - PubMed (original) (raw)
Characterization of a new 60 kDa apical protein of Plasmodium falciparum merozoite expressed in late schizogony
P Grellier et al. Biol Cell. 1994.
Abstract
Immunological cross-reactivity studies between the Apicomplexa Babesia divergens and Plasmodium falciparum allowed us to identify a P falciparum 60 kDa protein (Pf60) using an antiserum directed against a B divergens 37 kDa culture-derived exoantigen. In immunofluorescence assays (IFA), Pf60 appears as a doublet of fluorescent spots associated to the apical pole of merozoites. The doublet co-locates with two rhoptry components: the protein RAP-1 and the 140/130/110 (105) kDa rhoptry protein complex suggesting the rhoptry location of Pf60. The biosynthesis of Pf60, established by labeling experiments with [35S]methionine on synchronized cultures, and by immunofluorescence detection, occurred during late schizogony. The physico-chemical properties of Pf60, the absence of identified precursor forms and the absence of co-precipitation with other proteins indicated a new class of rhoptry protein. Pf60 was detected in all the different geographic P falciparum strains so far tested, with a slight variability in molecular mass ranging from 58 to 60 kDa. During the invasion process of erythrocytes by merozoites, the IFA showed the presence of the Pf60 in the apex of free merozoites, but not in invading merozoite, as well as in new ring-infected erythrocytes. Furthermore, immunoprecipitation assays indicated the presence of Pf60 in the culture medium, and its absence in new ring-infected erythrocytes. All together these results suggest a possible involvement of the Pf60 protein in the invasion process.
Similar articles
- Differential localization of full-length and processed forms of PF83/AMA-1 an apical membrane antigen of Plasmodium falciparum merozoites.
Narum DL, Thomas AW. Narum DL, et al. Mol Biochem Parasitol. 1994 Sep;67(1):59-68. doi: 10.1016/0166-6851(94)90096-5. Mol Biochem Parasitol. 1994. PMID: 7838184 - Identification and characterisation of proteins associated with the rhoptry organelles of Plasmodium falciparum merozoites.
Clark JT, Anand R, Akoglu T, McBride JS. Clark JT, et al. Parasitol Res. 1987;73(5):425-34. doi: 10.1007/BF00538200. Parasitol Res. 1987. PMID: 3309942 - Antibodies against a Plasmodium falciparum RON12 inhibit merozoite invasion into erythrocytes.
Ito D, Takashima E, Yamasaki T, Hatano S, Hasegawa T, Miura K, Morita M, Thongkukiatkul A, Diakite M, Long CA, Sattabongkot J, Udomsangpetch R, Iriko H, Ishino T, Tsuboi T. Ito D, et al. Parasitol Int. 2019 Feb;68(1):87-91. doi: 10.1016/j.parint.2018.10.006. Epub 2018 Oct 17. Parasitol Int. 2019. PMID: 30342119 Free PMC article. - Plasmodial ortholog of Toxoplasma gondii rhoptry neck protein 3 is localized to the rhoptry body.
Ito D, Han ET, Takeo S, Thongkukiatkul A, Otsuki H, Torii M, Tsuboi T. Ito D, et al. Parasitol Int. 2011 Jun;60(2):132-8. doi: 10.1016/j.parint.2011.01.001. Epub 2011 Jan 13. Parasitol Int. 2011. PMID: 21237287 - Identification of a Novel RAMA/RON3 Rhoptry Protein Complex in Plasmodium falciparum Merozoites.
Ito D, Chen JH, Takashima E, Hasegawa T, Otsuki H, Takeo S, Thongkukiatkul A, Han ET, Tsuboi T. Ito D, et al. Front Cell Infect Microbiol. 2021 Jan 18;10:605367. doi: 10.3389/fcimb.2020.605367. eCollection 2020. Front Cell Infect Microbiol. 2021. PMID: 33537242 Free PMC article.
Cited by
- Molecular aspects of severe malaria.
Chen Q, Schlichtherle M, Wahlgren M. Chen Q, et al. Clin Microbiol Rev. 2000 Jul;13(3):439-50. doi: 10.1128/CMR.13.3.439. Clin Microbiol Rev. 2000. PMID: 10885986 Free PMC article. Review.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Miscellaneous