The application of antisense oligonucleotide technology to the brain: some pitfalls - PubMed (original) (raw)
The application of antisense oligonucleotide technology to the brain: some pitfalls
B J Chiasson et al. Cell Mol Neurobiol. 1994 Oct.
Abstract
1. Amphetamine-induced c-fos and egr-1 expression in the striatum was used as a model in which to study the effects of antisense oligodeoxynucleotides (ODNs) directed at c-fos. Using direct infusions of ODNs into the striata of animals we have demonstrated that c-fos antisense ODNs retain most of their biological activity with 2- or 3-base substitutions. The c-fos antisense and mismatch ODNs attenuated Fos immunoreactivity but had little effect on Egr-1 immunoreactivity. 2. In another group of studies examining the role of c-fos in amygdala kindling, we have demonstrated that ODNs cause neurotoxic damage following repeated daily infusions into the amygdala. The damage observed was greatly diminished when the time interval between infusions was extended.
Similar articles
- Intra-amygdala infusion of an end-capped antisense oligodeoxynucleotide to c-fos accelerates amygdala kindling.
Chiasson BJ, Hong MG, Robertson HA. Chiasson BJ, et al. Brain Res Mol Brain Res. 1998 Jun 15;57(2):248-56. doi: 10.1016/s0169-328x(98)00090-4. Brain Res Mol Brain Res. 1998. PMID: 9675423 - Coordinate suppression of striatal ngfi-a and c-fos produces locomotor asymmetry and up-regulation of IEGs in the globus pallidus.
Hebb MO, Robertson HA. Hebb MO, et al. Brain Res Mol Brain Res. 1997 Aug;48(1):97-106. doi: 10.1016/s0169-328x(97)00086-7. Brain Res Mol Brain Res. 1997. PMID: 9379855 - In vivo administration of c-Fos antisense oligonucleotides accelerates amygdala kindling.
Rocha L, Kaufman DL. Rocha L, et al. Neurosci Lett. 1998 Jan 30;241(2-3):111-4. doi: 10.1016/s0304-3940(98)00002-0. Neurosci Lett. 1998. PMID: 9507933 - Putative roles for the inducible transcription factor c-fos in the central nervous system: studies with antisense oligonucleotides.
Chiasson BJ, Hong MG, Robertson HA. Chiasson BJ, et al. Neurochem Int. 1997 Sep;31(3):459-75. doi: 10.1016/s0197-0186(96)00115-5. Neurochem Int. 1997. PMID: 9246687 Review. - On the role of c-fos expression in striatal transmission. The antisense oligonucleotide approach.
Sommer W, Fuxe K. Sommer W, et al. Neurochem Int. 1997 Sep;31(3):425-36. doi: 10.1016/s0197-0186(96)00112-x. Neurochem Int. 1997. PMID: 9246684 Review.
Cited by
- Antisense Oligonucleotides: An Emerging Area in Drug Discovery and Development.
Dhuri K, Bechtold C, Quijano E, Pham H, Gupta A, Vikram A, Bahal R. Dhuri K, et al. J Clin Med. 2020 Jun 26;9(6):2004. doi: 10.3390/jcm9062004. J Clin Med. 2020. PMID: 32604776 Free PMC article. Review. - Antisense-mediated isoform switching of steroid receptor coactivator-1 in the central nucleus of the amygdala of the mouse brain.
Zalachoras I, Grootaers G, van Weert LT, Aubert Y, de Kreij SR, Datson NA, van Roon-Mom WM, Aartsma-Rus A, Meijer OC. Zalachoras I, et al. BMC Neurosci. 2013 Jan 7;14:5. doi: 10.1186/1471-2202-14-5. BMC Neurosci. 2013. PMID: 23294837 Free PMC article. - Stimulation of the dorsal periaqueductal gray enhances spontaneous recovery of a conditioned taste aversion.
Mickley GA, Ketchesin KD, Ramos L, Luchsinger JR, Rogers MM, Wiles NR, Hoxha N. Mickley GA, et al. Brain Res. 2013 Feb 1;1493:27-39. doi: 10.1016/j.brainres.2012.11.029. Epub 2012 Nov 23. Brain Res. 2013. PMID: 23183042 Free PMC article. - Antisense-mediated RNA targeting: versatile and expedient genetic manipulation in the brain.
Zalachoras I, Evers MM, van Roon-Mom WM, Aartsma-Rus AM, Meijer OC. Zalachoras I, et al. Front Mol Neurosci. 2011 Jul 19;4:10. doi: 10.3389/fnmol.2011.00010. eCollection 2011. Front Mol Neurosci. 2011. PMID: 21811437 Free PMC article. - A1 receptor and adenosinergic homeostatic regulation of sleep-wakefulness: effects of antisense to the A1 receptor in the cholinergic basal forebrain.
Thakkar MM, Winston S, McCarley RW. Thakkar MM, et al. J Neurosci. 2003 May 15;23(10):4278-87. doi: 10.1523/JNEUROSCI.23-10-04278.2003. J Neurosci. 2003. PMID: 12764116 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Other Literature Sources