Membrane-bound LERK2 ligand can signal through three different Eph-related receptor tyrosine kinases - PubMed (original) (raw)
Comparative Study
Membrane-bound LERK2 ligand can signal through three different Eph-related receptor tyrosine kinases
R Brambilla et al. EMBO J. 1995.
Abstract
The Eph-related family of receptor tyrosine kinases consists of at least 13 members, several of which display distinctive expression patterns in the developing and adult nervous system. Recently, a small family of ligands, structurally related to the B61 protein, was identified. Binding of these ligands to Eph-related receptors did not, however, elicit measurable biological signals in cultured cells. In order to study functional interactions between B61-related ligands and Eph-related receptors, we constructed chimeric receptors, containing an Eph-related ectodomain and the cytoplasmic domain of the TrkB neurotrophin receptor. Expression and activation of such chimeric receptors in NIH 3T3 cells induced transformation in focus formation assays. Membrane-bound LERK2 ligand is shown to signal through three different Eph-related receptors, namely Cek5, Cek10 and Elk. LERK2, however, fails to interact functionally with the Cek9 receptor. Quantitative analysis including binding assays indicates that Cek10 is the preferred LERK2 receptor. Preliminary mutagenesis of the LERK2 protein suggests a negative regulatory role for its cytoplasmic domain in LERK2 signaling.
Similar articles
- Tyrosine phosphorylation of transmembrane ligands for Eph receptors.
Brückner K, Pasquale EB, Klein R. Brückner K, et al. Science. 1997 Mar 14;275(5306):1640-3. doi: 10.1126/science.275.5306.1640. Science. 1997. PMID: 9054357 - Similarities and differences in the way transmembrane-type ligands interact with the Elk subclass of Eph receptors.
Brambilla R, Brückner K, Orioli D, Bergemann AD, Flanagan JG, Klein R. Brambilla R, et al. Mol Cell Neurosci. 1996;8(2-3):199-209. doi: 10.1006/mcne.1996.0057. Mol Cell Neurosci. 1996. PMID: 8918835 - The N-terminal globular domain of Eph receptors is sufficient for ligand binding and receptor signaling.
Labrador JP, Brambilla R, Klein R. Labrador JP, et al. EMBO J. 1997 Jul 1;16(13):3889-97. doi: 10.1093/emboj/16.13.3889. EMBO J. 1997. PMID: 9233799 Free PMC article. - Signaling by Eph receptors and their ephrin ligands.
Brückner K, Klein R. Brückner K, et al. Curr Opin Neurobiol. 1998 Jun;8(3):375-82. doi: 10.1016/s0959-4388(98)80064-0. Curr Opin Neurobiol. 1998. PMID: 9687349 Review. - Eph receptors and ephrins: regulators of guidance and assembly.
Wilkinson DG. Wilkinson DG. Int Rev Cytol. 2000;196:177-244. doi: 10.1016/s0074-7696(00)96005-4. Int Rev Cytol. 2000. PMID: 10730216 Review.
Cited by
- Contrasting Effects of Cancer-Associated Mutations in EphA3 and EphB2 Kinases.
Kim Y, Miller WT. Kim Y, et al. Biochemistry. 2024 Jan 22:10.1021/acs.biochem.3c00674. doi: 10.1021/acs.biochem.3c00674. Online ahead of print. Biochemistry. 2024. PMID: 38252844 - The Value of EphB2 Receptor and Cognate Ephrin Ligands in Prognostic and Predictive Assessments of Human Breast Cancer.
Ebrahim AS, Hailat Z, Bandyopadhyay S, Neill D, Kandouz M. Ebrahim AS, et al. Int J Mol Sci. 2021 Jul 28;22(15):8098. doi: 10.3390/ijms22158098. Int J Mol Sci. 2021. PMID: 34360867 Free PMC article. - EphrinB2-EphB4-RASA1 Signaling in Human Cerebrovascular Development and Disease.
Zeng X, Hunt A, Jin SC, Duran D, Gaillard J, Kahle KT. Zeng X, et al. Trends Mol Med. 2019 Apr;25(4):265-286. doi: 10.1016/j.molmed.2019.01.009. Epub 2019 Feb 25. Trends Mol Med. 2019. PMID: 30819650 Free PMC article. Review. - The coexpression of EphB4 and EphrinB2 is associated with poor prognosis in HER2-positive breast cancer.
Li X, Song C, Huang G, Sun S, Qiao J, Zhao J, Zhao Z, Li M. Li X, et al. Onco Targets Ther. 2017 Mar 21;10:1735-1742. doi: 10.2147/OTT.S132806. eCollection 2017. Onco Targets Ther. 2017. PMID: 28356761 Free PMC article. Retracted. - EphB3 Stimulates Cell Migration and Metastasis in a Kinase-dependent Manner through Vav2-Rho GTPase Axis in Papillary Thyroid Cancer.
Li JJ, Sun ZJ, Yuan YM, Yin FF, Bian YG, Long LY, Zhang XL, Xie D. Li JJ, et al. J Biol Chem. 2017 Jan 20;292(3):1112-1121. doi: 10.1074/jbc.M116.750349. Epub 2016 Dec 16. J Biol Chem. 2017. PMID: 27986811 Free PMC article.
References
- J Virol. 1969 Nov;4(5):549-53 - PubMed
- Oncogene. 1993 Aug;8(8):2033-42 - PubMed
- EMBO J. 1993 Aug;12(8):3083-94 - PubMed
- Oncogene. 1993 Oct;8(10):2857-62 - PubMed
- Mol Cell Biol. 1993 Nov;13(11):7071-9 - PubMed
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Miscellaneous