Positive autoregulation of the yeast transcription factor Pdr3p, which is involved in control of drug resistance - PubMed (original) (raw)
Positive autoregulation of the yeast transcription factor Pdr3p, which is involved in control of drug resistance
A Delahodde et al. Mol Cell Biol. 1995 Aug.
Abstract
Simultaneous resistance to an array of drugs with different cytotoxic activities is a property of Saccharomyces cerevisiae, in which the protein Pdr3p has recently been shown to play a role as a transcriptional regulator. We provide evidence that the yeast PDR3 gene, which encodes a zinc finger transcription factor implicated in certain drug resistance phenomena, is under positive autoregulation by Pdr3p. DNase I footprinting analyses using bacterially expressed Pdr3p showed specific recognition by this protein of at least two upstream activating sequences in the PDR3 promoter. The use of lacZ reporter constructs, a mutational analysis of the upstream activating sequences, as well as band shift experiments enabled the identification of two 5'TC CGCGGA3' sequence motifs in the PDR3 gene as consensus elements for the binding of Pdr3p. Several similar sequence motifs can be found in the promoter of PDR5, a gene encoding an ATP-dependent drug pump whose Pdr3p-induced overexpression is responsible for drug resistance phenomena. Recently one of these sequence elements was shown to be the target of Pdr3p to elevate the level of PDR5 transcription. Finally, we provide evidence in the absence of PDR1 for a PDR3-controlled transcriptional induction of the drug pump by cycloheximide and propose a model for the mechanism governing the transcriptional autoregulation of Pdr3p.
Similar articles
- Clustered amino acid substitutions in the yeast transcription regulator Pdr3p increase pleiotropic drug resistance and identify a new central regulatory domain.
Nourani A, Papajova D, Delahodde A, Jacq C, Subik J. Nourani A, et al. Mol Gen Genet. 1997 Oct;256(4):397-405. doi: 10.1007/s004380050583. Mol Gen Genet. 1997. PMID: 9393437 - Transcriptional control of the yeast PDR5 gene by the PDR3 gene product.
Katzmann DJ, Burnett PE, Golin J, Mahé Y, Moye-Rowley WS. Katzmann DJ, et al. Mol Cell Biol. 1994 Jul;14(7):4653-61. doi: 10.1128/mcb.14.7.4653-4661.1994. Mol Cell Biol. 1994. PMID: 8007969 Free PMC article. - Multiple Pdr1p/Pdr3p binding sites are essential for normal expression of the ATP binding cassette transporter protein-encoding gene PDR5.
Katzmann DJ, Hallstrom TC, Mahé Y, Moye-Rowley WS. Katzmann DJ, et al. J Biol Chem. 1996 Sep 20;271(38):23049-54. doi: 10.1074/jbc.271.38.23049. J Biol Chem. 1996. PMID: 8798494 - Yeast multidrug resistance: the PDR network.
Balzi E, Goffeau A. Balzi E, et al. J Bioenerg Biomembr. 1995 Feb;27(1):71-6. doi: 10.1007/BF02110333. J Bioenerg Biomembr. 1995. PMID: 7629054 Review.
Cited by
- Multilevel Regulation of Membrane Proteins in Response to Metal and Metalloid Stress: A Lesson from Yeast.
Zbieralski K, Staszewski J, Konczak J, Lazarewicz N, Nowicka-Kazmierczak M, Wawrzycka D, Maciaszczyk-Dziubinska E. Zbieralski K, et al. Int J Mol Sci. 2024 Apr 18;25(8):4450. doi: 10.3390/ijms25084450. Int J Mol Sci. 2024. PMID: 38674035 Free PMC article. Review. - Activity of the pleiotropic drug resistance transcription factors Pdr1p and Pdr3p is modulated by binding site flanking sequences.
Buechel ER, Pinkett HW. Buechel ER, et al. FEBS Lett. 2024 Jan;598(2):169-186. doi: 10.1002/1873-3468.14762. Epub 2023 Nov 8. FEBS Lett. 2024. PMID: 37873734 - De novo biosynthesis of rubusoside and rebaudiosides in engineered yeasts.
Xu Y, Wang X, Zhang C, Zhou X, Xu X, Han L, Lv X, Liu Y, Liu S, Li J, Du G, Chen J, Ledesma-Amaro R, Liu L. Xu Y, et al. Nat Commun. 2022 Jun 1;13(1):3040. doi: 10.1038/s41467-022-30826-2. Nat Commun. 2022. PMID: 35650215 Free PMC article. - SWI/SNF and the histone chaperone Rtt106 drive expression of the Pleiotropic Drug Resistance network genes.
Nikolov VN, Malavia D, Kubota T. Nikolov VN, et al. Nat Commun. 2022 Apr 12;13(1):1968. doi: 10.1038/s41467-022-29591-z. Nat Commun. 2022. PMID: 35413952 Free PMC article. - UME6 Is Involved in the Suppression of Basal Transcription of ABC Transporters and Drug Resistance in the ρ+ Cells of Saccharomyces cerevisiae.
Yamada Y. Yamada Y. Microorganisms. 2022 Mar 10;10(3):601. doi: 10.3390/microorganisms10030601. Microorganisms. 2022. PMID: 35336175 Free PMC article.
References
- Electrophoresis. 1993 Apr;14(4):259-65 - PubMed
- Mol Gen Genet. 1994 Sep 1;244(5):501-11 - PubMed
- J Biol Chem. 1993 Sep 5;268(25):18850-8 - PubMed
- Genes Dev. 1993 Sep;7(9):1824-35 - PubMed
- Mol Cell Biol. 1993 Nov;13(11):6941-56 - PubMed
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Medical
Molecular Biology Databases