B-cell-specific DNA binding by an E47 homodimer - PubMed (original) (raw)

B-cell-specific DNA binding by an E47 homodimer

C P Shen et al. Mol Cell Biol. 1995 Aug.

Abstract

B cells express a unique E-box-binding activity that contains basic helix-loop-helix (bHLH) proteins encoded by the E2A gene. E2A proteins play a central role in immunoglobulin gene transcription and are also required for the generation of the B-lymphocyte lineage. In muscle, E2A proteins bind DNA as heterodimers with muscle-specific bHLH partners, such as MyoD and myogenin, and these heterodimers are thought to be both necessary and sufficient for muscle determination in cultured cells. Our results indicate that in B cells, the bHLH partners for E2A proteins are not B-cell-restricted proteins, but are the E2A proteins themselves. UV cross-linking, gel purification, and the analysis of "forced heterodimers" indicate that BCF1 is primarily a homodimer of the E2A protein E47. Since E47 is widely expressed, our results argue for a difference in the inherent DNA-binding properties of the E47 protein in B cells and may help explain the restricted B-lineage defect observed in E2A-deficient mice.

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References

    1. Mol Endocrinol. 1994 Feb;8(2):197-209 - PubMed
    1. Cell. 1993 Dec 31;75(7):1351-9 - PubMed
    1. Genes Dev. 1994 Sep 15;8(18):2203-11 - PubMed
    1. Cell. 1994 Dec 2;79(5):875-84 - PubMed
    1. Cell. 1994 Dec 2;79(5):885-92 - PubMed

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