Isolation of multiple sequences from the Plasmodium falciparum genome that encode conserved domains homologous to those in erythrocyte-binding proteins - PubMed (original) (raw)

Comparative Study

Isolation of multiple sequences from the Plasmodium falciparum genome that encode conserved domains homologous to those in erythrocyte-binding proteins

D S Peterson et al. Proc Natl Acad Sci U S A. 1995.

Abstract

Open reading frames in the Plasmodium falciparum genome encode domains homologous to the adhesive domains of the P. falciparum EBA-175 erythrocyte-binding protein (eba-175 gene product) and those of the Plasmodium vivax and Plasmodium knowlesi Duffy antigen-binding proteins. These domains are referred to as Duffy binding-like (DBL), after the receptor that determines P. vivax invasion of Duffy blood group-positive human erythrocytes. Using oligonucleotide primers derived from short regions of conserved sequence, we have developed a reverse transcription-PCR method that amplifies sequences encoding the DBL domains of expressed genes. Products of these reverse transcription-PCR amplifications include sequences of single-copy genes (including eba-175) and variably transcribed genes that cross-hybridize to multiple regions of the genome. Restriction patterns of the multicopy genes show a high degree of polymorphism among different parasite lines, whereas single-copy genes are generally conserved. Characterization of the single-copy genes has identified a gene (ebl-1) that is related to eba-175 and is likely to be involved in erythrocyte invasion.

PubMed Disclaimer

Cited by

Expression of var genes located within polymorphic subtelomeric domains of Plasmodium falciparum chromosomes.
Fischer K, Horrocks P, Preuss M, Wiesner J, Wünsch S, Camargo AA, Lanzer M. Fischer K, et al. Mol Cell Biol. 1997 Jul;17(7):3679-86. doi: 10.1128/MCB.17.7.3679. Mol Cell Biol. 1997. PMID: 9199301 Free PMC article.
The adhesion of Plasmodium falciparum-infected erythrocytes to chondroitin sulfate A is mediated by P. falciparum erythrocyte membrane protein 1.
Reeder JC, Cowman AF, Davern KM, Beeson JG, Thompson JK, Rogerson SJ, Brown GV. Reeder JC, et al. Proc Natl Acad Sci U S A. 1999 Apr 27;96(9):5198-202. doi: 10.1073/pnas.96.9.5198. Proc Natl Acad Sci U S A. 1999. PMID: 10220443 Free PMC article.
Patterns of gene recombination shape var gene repertoires in Plasmodium falciparum: comparisons of geographically diverse isolates.
Kraemer SM, Kyes SA, Aggarwal G, Springer AL, Nelson SO, Christodoulou Z, Smith LM, Wang W, Levin E, Newbold CI, Myler PJ, Smith JD. Kraemer SM, et al. BMC Genomics. 2007 Feb 7;8:45. doi: 10.1186/1471-2164-8-45. BMC Genomics. 2007. PMID: 17286864 Free PMC article.
Cerebral Plasmodium falciparum malaria: The role of PfEMP1 in its pathogenesis and immunity, and PfEMP1-based vaccines to prevent it.
Jensen AR, Adams Y, Hviid L. Jensen AR, et al. Immunol Rev. 2020 Jan;293(1):230-252. doi: 10.1111/imr.12807. Epub 2019 Sep 27. Immunol Rev. 2020. PMID: 31562653 Free PMC article. Review.
Specific erythrocyte binding capacity and biological activity of Plasmodium falciparum erythrocyte binding ligand 1 (EBL-1)-derived peptides.
Curtidor H, Rodríguez LE, Ocampo M, López R, García JE, Valbuena J, Vera R, Puentes A, Vanegas M, Patarroyo ME. Curtidor H, et al. Protein Sci. 2005 Feb;14(2):464-73. doi: 10.1110/ps.041084305. Protein Sci. 2005. PMID: 15659376 Free PMC article.

References

1. Proteins. 1991;9(3):180-90 - PubMed
1. J Mol Biol. 1990 Oct 5;215(3):403-10 - PubMed
1. Proc Natl Acad Sci U S A. 1992 Aug 1;89(15):7085-9 - PubMed
1. Methods Mol Biol. 1993;21:319-32 - PubMed
1. Mol Biochem Parasitol. 1993 Sep;61(1):137-42 - PubMed

Isolation of multiple sequences from the Plasmodium falciparum genome that encode conserved domains homologous to those in erythrocyte-binding proteins - PubMed (original) (raw)