Characterization and purification of a primitive hematopoietic cell type in adult mouse marrow capable of lymphomyeloid differentiation in long-term marrow "switch" cultures - PubMed (original) (raw)
. 1995 Aug 15;86(4):1339-47.
Affiliations
- PMID: 7632940
Free article
Characterization and purification of a primitive hematopoietic cell type in adult mouse marrow capable of lymphomyeloid differentiation in long-term marrow "switch" cultures
M E Lemieux et al. Blood. 1995.
Free article
Abstract
In this report, we describe a modification of the assay for long-term culture-initiating cells (LTC-IC) that allows a subset of murine LTC-IC (designated as LTC-ICML) to express both their myeloid (M) and lymphoid (L) differentiative potentials in vitro. The modified assay involves culturing test cells at limiting dilutions on irradiated mouse marrow feeder layers for an initial 4 weeks under conditions that support myelopoiesis and then for an additional week under conditions permissive for B-lymphopoiesis. All of the clonogenic pre-B progenitors (colony-forming unit [CFU] pre-B) detected in such postswitch LTC appear to be the progeny of uncommitted cells present in the original cell suspension because exposure of lymphoid-restricted progenitors to myeloid LTC conditions for > or = 7 days was found to irreversibly terminate CFU-pre-B production and, in cultures initiated with limiting numbers of input cells (no progenitors of any type detected in > 70% of cultures 1 week after the switch), the presence of CFU-pre-B was tightly associated with the presence of myeloid clonogenic cells, regardless of the purity of the input population. Limiting dilution analysis of the proportion of negative cultures measured for different numbers of input cells showed the frequency of LTC-ICML in normal adult mouse marrow to be 1 per 5 x 10(5) cells with an enrichment of approximately 500-fold in the Sca-1+ Lin-WGA+ fraction, as was also found for competitive in vivo repopulating units (CRU) and conventionally defined LTC-IC. LTC-ICML also exhibited the same resistance to treatment in vivo with 5-fluorouracil (5-FU) as CRU and LTC-IC, thereby distinguishing these three populations from the great majority of both in vitro clonogenic cells and day 12 CFU-S. The ability to quantitate cells with dual lymphoid and myeloid differentiation potentials in vitro, without the need for their prior purification, should facilitate studies of totipotent hematopoietic stem cell regulation.
Similar articles
- Studies of W mutant mice provide evidence for alternate mechanisms capable of activating hematopoietic stem cells.
Miller CL, Rebel VI, Lemieux ME, Helgason CD, Lansdorp PM, Eaves CJ. Miller CL, et al. Exp Hematol. 1996 Feb;24(2):185-94. Exp Hematol. 1996. PMID: 8641340 - In vitro and in vivo evidence for the long-term multilineage (myeloid, B, NK, and T) reconstitution capacity of ex vivo expanded human CD34(+) cord blood cells.
Kobari L, Pflumio F, Giarratana M, Li X, Titeux M, Izac B, Leteurtre F, Coulombel L, Douay L. Kobari L, et al. Exp Hematol. 2000 Dec;28(12):1470-80. doi: 10.1016/s0301-472x(00)00557-9. Exp Hematol. 2000. PMID: 11146169 - Characterization of primitive hematopoietic cells in normal human peripheral blood.
Udomsakdi C, Lansdorp PM, Hogge DE, Reid DS, Eaves AC, Eaves CJ. Udomsakdi C, et al. Blood. 1992 Nov 15;80(10):2513-21. Blood. 1992. PMID: 1384786 - Marrow Hematopoietic Stem Cells Revisited: They Exist in a Continuum and are Not Defined by Standard Purification Approaches; Then There are the Microvesicles.
Quesenberry PJ, Goldberg L, Aliotta J, Dooner M. Quesenberry PJ, et al. Front Oncol. 2014 Apr 4;4:56. doi: 10.3389/fonc.2014.00056. eCollection 2014. Front Oncol. 2014. PMID: 24772390 Free PMC article. Review.
Cited by
- ROS-mediated amplification of AKT/mTOR signalling pathway leads to myeloproliferative syndrome in Foxo3(-/-) mice.
Yalcin S, Marinkovic D, Mungamuri SK, Zhang X, Tong W, Sellers R, Ghaffari S. Yalcin S, et al. EMBO J. 2010 Dec 15;29(24):4118-31. doi: 10.1038/emboj.2010.292. Epub 2010 Nov 26. EMBO J. 2010. PMID: 21113129 Free PMC article. - The RARγ Oncogene: An Achilles Heel for Some Cancers.
Brown G, Petrie K. Brown G, et al. Int J Mol Sci. 2021 Mar 31;22(7):3632. doi: 10.3390/ijms22073632. Int J Mol Sci. 2021. PMID: 33807298 Free PMC article. Review. - Restraining Lysosomal Activity Preserves Hematopoietic Stem Cell Quiescence and Potency.
Liang R, Arif T, Kalmykova S, Kasianov A, Lin M, Menon V, Qiu J, Bernitz JM, Moore K, Lin F, Benson DL, Tzavaras N, Mahajan M, Papatsenko D, Ghaffari S. Liang R, et al. Cell Stem Cell. 2020 Mar 5;26(3):359-376.e7. doi: 10.1016/j.stem.2020.01.013. Epub 2020 Feb 27. Cell Stem Cell. 2020. PMID: 32109377 Free PMC article. - Proliferation of multipotent hematopoietic cells controlled by a truncated erythropoietin receptor transgene.
Kirby SL, Cook DN, Walton W, Smithies O. Kirby SL, et al. Proc Natl Acad Sci U S A. 1996 Sep 3;93(18):9402-7. doi: 10.1073/pnas.93.18.9402. Proc Natl Acad Sci U S A. 1996. PMID: 8790342 Free PMC article. - Expansion in vitro of transplantable human cord blood stem cells demonstrated using a quantitative assay of their lympho-myeloid repopulating activity in nonobese diabetic-scid/scid mice.
Conneally E, Cashman J, Petzer A, Eaves C. Conneally E, et al. Proc Natl Acad Sci U S A. 1997 Sep 2;94(18):9836-41. doi: 10.1073/pnas.94.18.9836. Proc Natl Acad Sci U S A. 1997. PMID: 9275212 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Medical
Research Materials