A family of Ran binding proteins that includes nucleoporins - PubMed (original) (raw)
A family of Ran binding proteins that includes nucleoporins
C Dingwall et al. Proc Natl Acad Sci U S A. 1995.
Abstract
Ran, a small nuclear GTP binding protein, is essential for the translocation of nuclear proteins through the nuclear pore complex. We show that several proteins, including the Saccharomyces cerevisiae Nup2p and Caenorhabditis elegans F59A2.1 nucleoporins, contain domains similar to the previously characterized murine Ran binding protein (RBP, termed RBP1). To test the significance of this similarity, we have used the corresponding domains of Nup2p and a putative S. cerevisiae RBP in Ran binding assays and the yeast two-hybrid system. Both proteins bind S. cerevisiae Ran, but only the putative S. cerevisiae RBP binds human Ran. Two-hybrid analysis revealed Ran-Ran interactions and that yeast and human Rans can interact. These data identify Nup2p as a target for Ran in the nuclear pore complex, suggesting a direct role for it in nuclear-cytoplasmic transport. We discuss the possibility that proteins harboring Ran binding domains link the Ran GTPase cycle to specific functions in the nucleus.
Similar articles
- Yrb4p, a yeast ran-GTP-binding protein involved in import of ribosomal protein L25 into the nucleus.
Schlenstedt G, Smirnova E, Deane R, Solsbacher J, Kutay U, Görlich D, Ponstingl H, Bischoff FR. Schlenstedt G, et al. EMBO J. 1997 Oct 15;16(20):6237-49. doi: 10.1093/emboj/16.20.6237. EMBO J. 1997. PMID: 9321403 Free PMC article. - Interactions between a nuclear transporter and a subset of nuclear pore complex proteins depend on Ran GTPase.
Seedorf M, Damelin M, Kahana J, Taura T, Silver PA. Seedorf M, et al. Mol Cell Biol. 1999 Feb;19(2):1547-57. doi: 10.1128/MCB.19.2.1547. Mol Cell Biol. 1999. PMID: 9891088 Free PMC article. - RNA1 encodes a GTPase-activating protein specific for Gsp1p, the Ran/TC4 homologue of Saccharomyces cerevisiae.
Becker J, Melchior F, Gerke V, Bischoff FR, Ponstingl H, Wittinghofer A. Becker J, et al. J Biol Chem. 1995 May 19;270(20):11860-5. doi: 10.1074/jbc.270.20.11860. J Biol Chem. 1995. PMID: 7744835 - Transport into and out of the nucleus.
Macara IG. Macara IG. Microbiol Mol Biol Rev. 2001 Dec;65(4):570-94, table of contents. doi: 10.1128/MMBR.65.4.570-594.2001. Microbiol Mol Biol Rev. 2001. PMID: 11729264 Free PMC article. Review. - The molecular mechanism of transport of macromolecules through nuclear pore complexes.
Bayliss R, Corbett AH, Stewart M. Bayliss R, et al. Traffic. 2000 Jun;1(6):448-56. doi: 10.1034/j.1600-0854.2000.010602.x. Traffic. 2000. PMID: 11208130 Review.
Cited by
- A T42A Ran mutation: differential interactions with effectors and regulators, and defect in nuclear protein import.
Murphy GA, Moore MS, Drivas G, Pérez de la Ossa P, Villamarin A, D'Eustachio P, Rush MG. Murphy GA, et al. Mol Biol Cell. 1997 Dec;8(12):2591-604. doi: 10.1091/mbc.8.12.2591. Mol Biol Cell. 1997. PMID: 9398678 Free PMC article. - Ran's C-terminal, basic patch, and nucleotide exchange mechanisms in light of a canonical structure for Rab, Rho, Ras, and Ran GTPases.
Neuwald AF, Kannan N, Poleksic A, Hata N, Liu JS. Neuwald AF, et al. Genome Res. 2003 Apr;13(4):673-92. doi: 10.1101/gr.862303. Genome Res. 2003. PMID: 12671004 Free PMC article. - A role for the divergent actin gene, ACT2, in nuclear pore structure and function.
Yan C, Leibowitz N, Mélèse T. Yan C, et al. EMBO J. 1997 Jun 16;16(12):3572-86. doi: 10.1093/emboj/16.12.3572. EMBO J. 1997. PMID: 9218799 Free PMC article. - When overexpressed, a novel centrosomal protein, RanBPM, causes ectopic microtubule nucleation similar to gamma-tubulin.
Nakamura M, Masuda H, Horii J, Kuma Ki, Yokoyama N, Ohba T, Nishitani H, Miyata T, Tanaka M, Nishimoto T. Nakamura M, et al. J Cell Biol. 1998 Nov 16;143(4):1041-52. doi: 10.1083/jcb.143.4.1041. J Cell Biol. 1998. PMID: 9817760 Free PMC article. - RanBP1 stabilizes the interaction of Ran with p97 nuclear protein import.
Chi NC, Adam EJ, Visser GD, Adam SA. Chi NC, et al. J Cell Biol. 1996 Nov;135(3):559-69. doi: 10.1083/jcb.135.3.559. J Cell Biol. 1996. PMID: 8909533 Free PMC article.
References
- EMBO J. 1994 Jun 15;13(12):2897-903 - PubMed
- Proc Natl Acad Sci U S A. 1994 Mar 29;91(7):2587-91 - PubMed
- Nature. 1989 Jul 20;340(6230):245-6 - PubMed
- Trends Cell Biol. 1994 Oct;4(10):357-65 - PubMed
- J Cell Biol. 1994 Aug;126(3):619-30 - PubMed
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Molecular Biology Databases
Miscellaneous