Identification of transcriptional start sites and the role of ppGpp in the expression of rpoS, the structural gene for the sigma S subunit of RNA polymerase in Escherichia coli - PubMed (original) (raw)

Identification of transcriptional start sites and the role of ppGpp in the expression of rpoS, the structural gene for the sigma S subunit of RNA polymerase in Escherichia coli

R Lange et al. J Bacteriol. 1995 Aug.

Abstract

rpoS is the structural gene for the sigma S subunit of RNA polymerase which controls the expression of a large number of genes in Escherichia coli that are induced during entry into stationary phase or in response to increased medium osmolarity. Using a combination of primer extension experiments and a 5' deletion analysis of the region upstream of rpoS, we show that rpoS transcription is mainly driven by a single promoter (rpoSp1) located within the nlpD gene upstream of rpoS (the two relatively weak nlpD promoters contribute to the low level of rpoS expression during early exponential phase). In addition, we demonstrate that the expression of both transcriptional and translational rpoS::lacZ fusions as well as the level of rpoS mRNA originating at rpoSp1 is strongly reduced in ppGpp-deficient relA spoT mutants. However, experiments with the 5' deletion constructs indicate that a lack of ppGpp does affect transcriptional elongation rather than initiation.

PubMed Disclaimer

Cited by

RpoS and the bacterial general stress response.
Bouillet S, Bauer TS, Gottesman S. Bouillet S, et al. Microbiol Mol Biol Rev. 2024 Mar 27;88(1):e0015122. doi: 10.1128/mmbr.00151-22. Epub 2024 Feb 27. Microbiol Mol Biol Rev. 2024. PMID: 38411096 Review.
Pleiotropic effects of a rel mutation on stress survival of Rhizobium etli CNPAF512.
Braeken K, Fauvart M, Vercruysse M, Beullens S, Lambrichts I, Michiels J. Braeken K, et al. BMC Microbiol. 2008 Dec 10;8:219. doi: 10.1186/1471-2180-8-219. BMC Microbiol. 2008. PMID: 19077212 Free PMC article.
Two (p)ppGpp Synthetase Genes, relA and spoT, Are Involved in Regulating Cell Motility, Exopolysaccharides Production, and Biofilm Formation of Vibrio alginolyticus.
Yin WL, Xie ZY, Zeng YH, Zhang J, Long H, Ren W, Zhang X, Cai XN, Huang AY. Yin WL, et al. Front Microbiol. 2022 Mar 29;13:858559. doi: 10.3389/fmicb.2022.858559. eCollection 2022. Front Microbiol. 2022. PMID: 35422789 Free PMC article.
Regulation of rpoS gene expression in Pseudomonas: involvement of a TetR family regulator.
Kojic M, Venturi V. Kojic M, et al. J Bacteriol. 2001 Jun;183(12):3712-20. doi: 10.1128/JB.183.12.3712-3720.2001. J Bacteriol. 2001. PMID: 11371535 Free PMC article.
The rpoS mRNA leader recruits Hfq to facilitate annealing with DsrA sRNA.
Soper TJ, Woodson SA. Soper TJ, et al. RNA. 2008 Sep;14(9):1907-17. doi: 10.1261/rna.1110608. Epub 2008 Jul 24. RNA. 2008. PMID: 18658123 Free PMC article.

References

1. J Bacteriol. 1993 Apr;175(7):2150-3 - PubMed
1. J Bacteriol. 1993 Apr;175(7):2143-9 - PubMed
1. J Bacteriol. 1994 Mar;176(6):1630-8 - PubMed
1. Mol Gen Genet. 1994 Jun 3;243(5):525-31 - PubMed
1. Biochim Biophys Acta. 1994 Oct 18;1219(2):425-34 - PubMed

Identification of transcriptional start sites and the role of ppGpp in the expression of rpoS, the structural gene for the sigma S subunit of RNA polymerase in Escherichia coli - PubMed (original) (raw)