Ascorbic acid enhances ferritin mRNA translation by an IRP/aconitase switch - PubMed (original) (raw)
. 1995 Aug 18;270(33):19540-4.
doi: 10.1074/jbc.270.33.19540.
Affiliations
- PMID: 7642638
- DOI: 10.1074/jbc.270.33.19540
Free article
Ascorbic acid enhances ferritin mRNA translation by an IRP/aconitase switch
I Toth et al. J Biol Chem. 1995.
Free article
Abstract
Replenishment of ascorbate in cultured cells, which are almost uniformly vitamin-deficient, increases ferritin mRNA translation in response to iron by 20-fold (Toth, I., Rogers, J. T., McPhee, J. A., Elliott, S. M., Abramson, S. L., and Bridges, K. R. (1995) J. Biol. Chem. 270, 2846-2852). We now demonstrate that ascorbate increases cytosolic aconitase activity. The iron-responsive element-binding protein (IRP-1) exists in three states: bound to mRNA without aconitase activity, free in the cytosol without aconitase activity, and free in the cytosol with aconitase activity. Ascorbate converts free IRP-1 to the enzymatically active form. Enhanced ferritin synthesis with subsequent iron stimulation is due to the altered equilibrium of the free IRP-1. The cellular biology of iron is closely intertwined with that of ascorbate.
Similar articles
- Dietary iron intake modulates the activity of iron regulatory proteins and the abundance of ferritin and mitochondrial aconitase in rat liver.
Chen OS, Schalinske KL, Eisenstein RS. Chen OS, et al. J Nutr. 1997 Feb;127(2):238-48. doi: 10.1093/jn/127.2.238. J Nutr. 1997. PMID: 9039823 - Recycling of RNA binding iron regulatory protein 1 into an aconitase after nitric oxide removal depends on mitochondrial ATP.
Bouton C, Chauveau MJ, Lazereg S, Drapier JC. Bouton C, et al. J Biol Chem. 2002 Aug 23;277(34):31220-7. doi: 10.1074/jbc.M203276200. Epub 2002 May 30. J Biol Chem. 2002. PMID: 12039960 - Binding of cytosolic aconitase to the iron responsive element of porcine mitochondrial aconitase mRNA.
Zheng L, Kennedy MC, Blondin GA, Beinert H, Zalkin H. Zheng L, et al. Arch Biochem Biophys. 1992 Dec;299(2):356-60. doi: 10.1016/0003-9861(92)90287-7. Arch Biochem Biophys. 1992. PMID: 1444477 - Regulation of iron metabolism in eukaryotes.
Rouault T, Klausner R. Rouault T, et al. Curr Top Cell Regul. 1997;35:1-19. doi: 10.1016/s0070-2137(97)80001-5. Curr Top Cell Regul. 1997. PMID: 9192174 Review. - An iron-sulfur cluster plays a novel regulatory role in the iron-responsive element binding protein.
Rouault TA, Haile DJ, Downey WE, Philpott CC, Tang C, Samaniego F, Chin J, Paul I, Orloff D, Harford JB, et al. Rouault TA, et al. Biometals. 1992 Autumn;5(3):131-40. doi: 10.1007/BF01061319. Biometals. 1992. PMID: 1421965 Review.
Cited by
- Crosstalk between regulated necrosis and micronutrition, bridged by reactive oxygen species.
Zhang L, Liu J, Dai Z, Wang J, Wu M, Su R, Zhang D. Zhang L, et al. Front Nutr. 2022 Sep 23;9:1003340. doi: 10.3389/fnut.2022.1003340. eCollection 2022. Front Nutr. 2022. PMID: 36211509 Free PMC article. Review. - Ferritin and the response to oxidative stress.
Orino K, Lehman L, Tsuji Y, Ayaki H, Torti SV, Torti FM. Orino K, et al. Biochem J. 2001 Jul 1;357(Pt 1):241-7. doi: 10.1042/0264-6021:3570241. Biochem J. 2001. PMID: 11415455 Free PMC article. - Iron-mediated degradation of IRP2, an unexpected pathway involving a 2-oxoglutarate-dependent oxygenase activity.
Wang J, Chen G, Muckenthaler M, Galy B, Hentze MW, Pantopoulos K. Wang J, et al. Mol Cell Biol. 2004 Feb;24(3):954-65. doi: 10.1128/MCB.24.3.954-965.2004. Mol Cell Biol. 2004. PMID: 14729944 Free PMC article. - Physiology and Inflammation Driven Pathophysiology of Iron Homeostasis-Mechanistic Insights into Anemia of Inflammation and Its Treatment.
Lanser L, Fuchs D, Kurz K, Weiss G. Lanser L, et al. Nutrients. 2021 Oct 22;13(11):3732. doi: 10.3390/nu13113732. Nutrients. 2021. PMID: 34835988 Free PMC article. Review. - An iron-dependent metabolic vulnerability underlies VPS34-dependence in RKO cancer cells.
Kobylarz MJ, Goodwin JM, Kang ZB, Annand JW, Hevi S, O'Mahony E, McAllister G, Reece-Hoyes J, Wang Q, Alford J, Russ C, Lindeman A, Beibel M, Roma G, Carbone W, Knehr J, Loureiro J, Antczak C, Wiederschain D, Murphy LO, Menon S, Nyfeler B. Kobylarz MJ, et al. PLoS One. 2020 Aug 24;15(8):e0235551. doi: 10.1371/journal.pone.0235551. eCollection 2020. PLoS One. 2020. PMID: 32833964 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Medical