A dimerization motif for transmembrane alpha-helices - PubMed (original) (raw)
A dimerization motif for transmembrane alpha-helices
M A Lemmon et al. Nat Struct Biol. 1994 Mar.
Abstract
Specific helix-helix interactions inside lipid bilayers guide the folding and assembly of many integral membrane proteins and their complexes. We report here a pattern of 7 amino acids (LIxxGVxxGVxxT) which when introduced into several hydrophobic transmembrane alpha-helices promotes their specific dimerization. Dimerization is driven by interactions that are specific, dominated by the helix-helix interface, and involve no potentially ionizable groups. The motif may provide a useful tool for the functional analysis of such interactions in a variety of systems. Further, since this particular motif is rare, whilst specific helix association is not, many other such motifs may exist, which could permit sorting within complex membranes as well as guiding folding and oligomerization.
Similar articles
- Sequence and conformational preferences at termini of α-helices in membrane proteins: role of the helix environment.
Shelar A, Bansal M. Shelar A, et al. Proteins. 2014 Dec;82(12):3420-36. doi: 10.1002/prot.24696. Epub 2014 Oct 10. Proteins. 2014. PMID: 25257385 - Sequence dependence of BNIP3 transmembrane domain dimerization implicates side-chain hydrogen bonding and a tandem GxxxG motif in specific helix-helix interactions.
Sulistijo ES, MacKenzie KR. Sulistijo ES, et al. J Mol Biol. 2006 Dec 15;364(5):974-90. doi: 10.1016/j.jmb.2006.09.065. Epub 2006 Sep 29. J Mol Biol. 2006. PMID: 17049556 - Transmembrane helix dimerization: beyond the search for sequence motifs.
Li E, Wimley WC, Hristova K. Li E, et al. Biochim Biophys Acta. 2012 Feb;1818(2):183-93. doi: 10.1016/j.bbamem.2011.08.031. Epub 2011 Sep 1. Biochim Biophys Acta. 2012. PMID: 21910966 Free PMC article. Review. - Transmembrane helix-helix interactions are modulated by the sequence context and by lipid bilayer properties.
Cymer F, Veerappan A, Schneider D. Cymer F, et al. Biochim Biophys Acta. 2012 Apr;1818(4):963-73. doi: 10.1016/j.bbamem.2011.07.035. Epub 2011 Jul 31. Biochim Biophys Acta. 2012. PMID: 21827736 Review. - Characterization of a membrane protein folding motif, the Ser zipper, using designed peptides.
North B, Cristian L, Fu Stowell X, Lear JD, Saven JG, Degrado WF. North B, et al. J Mol Biol. 2006 Jun 16;359(4):930-9. doi: 10.1016/j.jmb.2006.04.001. Epub 2006 Apr 19. J Mol Biol. 2006. PMID: 16697010
Cited by
- A Rigorous Framework for Calculating Protein-Protein Binding Affinities in Membranes.
Blazhynska M, Gumbart JC, Chen H, Tajkhorshid E, Roux B, Chipot C. Blazhynska M, et al. J Chem Theory Comput. 2023 Dec 26;19(24):9077-9092. doi: 10.1021/acs.jctc.3c00941. Epub 2023 Dec 13. J Chem Theory Comput. 2023. PMID: 38091976 - Structural Determinant of β-Amyloid Formation: From Transmembrane Protein Dimerization to β-Amyloid Aggregates.
Papadopoulos N, Suelves N, Perrin F, Vadukul DM, Vrancx C, Constantinescu SN, Kienlen-Campard P. Papadopoulos N, et al. Biomedicines. 2022 Oct 29;10(11):2753. doi: 10.3390/biomedicines10112753. Biomedicines. 2022. PMID: 36359274 Free PMC article. Review. - Activation of STING Based on Its Structural Features.
Hussain B, Xie Y, Jabeen U, Lu D, Yang B, Wu C, Shang G. Hussain B, et al. Front Immunol. 2022 Jul 19;13:808607. doi: 10.3389/fimmu.2022.808607. eCollection 2022. Front Immunol. 2022. PMID: 35928815 Free PMC article. Review. - Applications and evolution of melittin, the quintessential membrane active peptide.
Guha S, Ferrie RP, Ghimire J, Ventura CR, Wu E, Sun L, Kim SY, Wiedman GR, Hristova K, Wimley WC. Guha S, et al. Biochem Pharmacol. 2021 Nov;193:114769. doi: 10.1016/j.bcp.2021.114769. Epub 2021 Sep 17. Biochem Pharmacol. 2021. PMID: 34543656 Free PMC article. Review. - Diverse Scientific Benchmarks for Implicit Membrane Energy Functions.
Alford RF, Samanta R, Gray JJ. Alford RF, et al. J Chem Theory Comput. 2021 Aug 10;17(8):5248-5261. doi: 10.1021/acs.jctc.0c00646. Epub 2021 Jul 26. J Chem Theory Comput. 2021. PMID: 34310137 Free PMC article.