Distribution and origin of the basement membrane component perlecan in rat liver and primary hepatocyte culture - PubMed (original) (raw)
. 1993 Jan;142(1):199-208.
Affiliations
- PMID: 7678718
- PMCID: PMC1886845
Distribution and origin of the basement membrane component perlecan in rat liver and primary hepatocyte culture
P Y Rescan et al. Am J Pathol. 1993 Jan.
Abstract
Basement membranes contain three major components (ie collagen IV, laminin, and the heparan sulfate proteoglycan termed perlecan). Although the distribution and origin of both collagen IV and laminin have been well documented in the liver, perlecan has been poorly investigated, so far. We have studied the distribution and cellular origin of perlecan in rat livers in various conditions as well as in hepatocyte primary culture. By immunolocalization in both adult and 18-day-old fetal liver, perlecan was found in portal spaces, around central veins, and throughout the lobule. Immunoelectron microscopy revealed its presence at the level of basement membranes surrounding bile ducts and blood vessels, and in the space of Disse discontinuously interacting with hepatocyte microvilli. Precursors of perlecan were detected in the rough endoplasmic reticulum of bile duct cells and both vascular and sinusoidal endothelial cells. Both hepatocytes and Ito cells were negative. Northern-blot analysis confirmed the lack of appreciable expression of perlecan in hepatocytes isolated from either fetal or adult livers. In 18-month-diethylnitrosamine-treated rat liver, perlecan was abundant in neoplastic nodules. Electron microscopic investigation revealed an almost continuous layer of perlecan in the space of Disse and intracellular staining in sinusoidal endothelial cells, only. Perlecan mRNAs were detectable in malignant nodules, and absent in hepatocytes from nontumorous areas. Hepatocytes expressed high levels of perlecan mRNAs only when put in culture. This expression was reduced in conditions that allow improvement of hepatocyte survival and function (ie addition of corticoids, dimethylsulfoxide or nicotinamide to the medium, or in coculture with liver epithelial cells from biliary origin). Immunolocalization by light and electron microscopy showed that deposition of the proteoglycan occurred in coculture, in basement membranelike structures located around hepatocyte cords. In vitro attachment assay of hepatocytes on purified perlecan substrate indicated that these cells may interact with the proteoglycan through integrins which belong to the beta 1 family. These data suggest that deposition of perlecan in the space of Disse requires cellular cooperation. This article on perlecan, the third major component of hepatic basement membranes, shows a unique cellular origin in the liver and, as found for both collagen IV and laminin, an expression in adult hepatocytes when place in culture.
Similar articles
- Differential expression of laminin chains and receptor (LBP-32) in fetal and neoplastic hepatocytes compared to normal adult hepatocytes in vivo and in culture.
Rescan PY, Clément B, Yamada Y, Segui-Real B, Baffet G, Guguen-Guillouzo C, Guillouzo A. Rescan PY, et al. Am J Pathol. 1990 Sep;137(3):701-9. Am J Pathol. 1990. PMID: 2144711 Free PMC article. - Heparan sulfate proteoglycan expression in normal human liver.
Roskams T, Moshage H, De Vos R, Guido D, Yap P, Desmet V. Roskams T, et al. Hepatology. 1995 Apr;21(4):950-8. Hepatology. 1995. PMID: 7705805 - Expression of extracellular matrix proteoglycans perlecan and decorin in carbon-tetrachloride-injured rat liver and in isolated liver cells.
Gallai M, Kovalszky I, Knittel T, Neubauer K, Armbrust T, Ramadori G. Gallai M, et al. Am J Pathol. 1996 May;148(5):1463-71. Am J Pathol. 1996. PMID: 8623917 Free PMC article. - Expression and potential role of the extracellular matrix in hepatic ontogenesis: a review.
Amenta PS, Harrison D. Amenta PS, et al. Microsc Res Tech. 1997 Nov 15;39(4):372-86. doi: 10.1002/(SICI)1097-0029(19971115)39:4<372::AID-JEMT7>3.0.CO;2-J. Microsc Res Tech. 1997. PMID: 9407547 Review. - [Morphology of basement membrane and associated matrix proteins in normal and pathological tissues].
Nerlich A. Nerlich A. Veroff Pathol. 1995;145:1-139. Veroff Pathol. 1995. PMID: 8638427 Review. German.
Cited by
- Decellularization Techniques for Tissue Engineering: Towards Replicating Native Extracellular Matrix Architecture in Liver Regeneration.
Allu I, Sahi AK, Koppadi M, Gundu S, Sionkowska A. Allu I, et al. J Funct Biomater. 2023 Oct 16;14(10):518. doi: 10.3390/jfb14100518. J Funct Biomater. 2023. PMID: 37888183 Free PMC article. Review. - Thorny ground, rocky soil: Tissue-specific mechanisms of tumor dormancy and relapse.
Lim AR, Ghajar CM. Lim AR, et al. Semin Cancer Biol. 2022 Jan;78:104-123. doi: 10.1016/j.semcancer.2021.05.007. Epub 2021 May 9. Semin Cancer Biol. 2022. PMID: 33979673 Free PMC article. Review. - Proteoglycans-Biomarkers and Targets in Cancer Therapy.
Nikitovic D, Berdiaki A, Spyridaki I, Krasanakis T, Tsatsakis A, Tzanakakis GN. Nikitovic D, et al. Front Endocrinol (Lausanne). 2018 Mar 6;9:69. doi: 10.3389/fendo.2018.00069. eCollection 2018. Front Endocrinol (Lausanne). 2018. PMID: 29559954 Free PMC article. Review. - Immune signatures of pathogenesis in the peritoneal compartment during early infection of sheep with Fasciola hepatica.
Ruiz-Campillo MT, Molina Hernandez V, Escamilla A, Stevenson M, Perez J, Martinez-Moreno A, Donnelly S, Dalton JP, Cwiklinski K. Ruiz-Campillo MT, et al. Sci Rep. 2017 Jun 5;7(1):2782. doi: 10.1038/s41598-017-03094-0. Sci Rep. 2017. PMID: 28584245 Free PMC article. - Hepatocyte growth factor in patients with coronary artery disease and its relation to periodontal condition.
Lönn J, Starkhammar Johansson C, Kälvegren H, Brudin L, Skoglund C, Garvin P, Särndahl E, Ravald N, Richter A, Bengtsson T, Nayeri F. Lönn J, et al. Results Immunol. 2011 Dec 30;2:7-12. doi: 10.1016/j.rinim.2011.12.002. eCollection 2011. Results Immunol. 2011. PMID: 24371561 Free PMC article.
References
- J Biol Chem. 1988 Nov 5;263(31):16379-87 - PubMed
- J Cell Biol. 1985 Mar;100(3):975-80 - PubMed
- Cell Differ Dev. 1989 Mar;26(2):131-44 - PubMed
- J Biol Chem. 1989 Jul 25;264(21):12467-71 - PubMed
- Am J Pathol. 1989 Jun;134(6):1175-82 - PubMed
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources