Amphiregulin induces tyrosine phosphorylation of the epidermal growth factor receptor and p185erbB2. Evidence that amphiregulin acts exclusively through the epidermal growth factor receptor at the surface of human epithelial cells - PubMed (original) (raw)
. 1993 Feb 5;268(4):2924-31.
Affiliations
- PMID: 7679104
Free article
Amphiregulin induces tyrosine phosphorylation of the epidermal growth factor receptor and p185erbB2. Evidence that amphiregulin acts exclusively through the epidermal growth factor receptor at the surface of human epithelial cells
G R Johnson et al. J Biol Chem. 1993.
Free article
Abstract
The COOH-terminal half of the amphiregulin (AR) molecule has sequence homology to epidermal growth factor (EGF). The ability of AR to elicit in vivo phosphorylation of the EGF receptor (EGFR) and p185erbB2 was studied in four human epithelial cell lines which expressed either or both of the receptor tyrosine kinases. AR induced the phosphorylation of the EGFR and p185erbB2, and phosphoamino acid analysis revealed enhanced phosphorylation of tyrosine residues in both receptor proteins. A monoclonal antibody (mAb) which binds to the extracellular domain of the EGFR blocked the phosphorylation of the EGFR and p185erbB2 as well as AR-induced mitogenesis indicating that the EGFR mediated these responses. In MDA-MB-453 cells which lack EGFRs, AR did not induce phosphorylation of p185erbB2, did not affect proliferation, and had no detectable effect on the phosphorylation of cellular proteins isolated using an anti-phosphotyrosine mAb. Qualitatively, in vivo phosphorylations induced by AR and EGF were found to be indistinguishable as demonstrated by analysis of cellular 32P-labeled proteins isolated with the anti-phosphotyrosine mAb. Moreover, in the presence of the anti-EGFR mAb, AR had no effect on the proliferation of cells. These results provide strong evidence that the EGFR is the sole cell surface mediator of the action of AR in human epithelial cells.
Similar articles
- The epidermal growth factor receptor couples transforming growth factor-alpha, heparin-binding epidermal growth factor-like factor, and amphiregulin to Neu, ErbB-3, and ErbB-4.
Riese DJ, Kim ED, Elenius K, Buckley S, Klagsbrun M, Plowman GD, Stern DF. Riese DJ, et al. J Biol Chem. 1996 Aug 16;271(33):20047-52. doi: 10.1074/jbc.271.33.20047. J Biol Chem. 1996. PMID: 8702723 - A differential requirement for the COOH-terminal region of the epidermal growth factor (EGF) receptor in amphiregulin and EGF mitogenic signaling.
Wong L, Deb TB, Thompson SA, Wells A, Johnson GR. Wong L, et al. J Biol Chem. 1999 Mar 26;274(13):8900-9. doi: 10.1074/jbc.274.13.8900. J Biol Chem. 1999. PMID: 10085134 - Autocrine action of amphiregulin in a colon carcinoma cell line and immunocytochemical localization of amphiregulin in human colon.
Johnson GR, Saeki T, Gordon AW, Shoyab M, Salomon DS, Stromberg K. Johnson GR, et al. J Cell Biol. 1992 Aug;118(3):741-51. doi: 10.1083/jcb.118.3.741. J Cell Biol. 1992. PMID: 1639855 Free PMC article. - The role of amphiregulin in breast cancer.
Salomon DS, Normanno N, Ciardiello F, Brandt R, Shoyab M, Todaro GJ. Salomon DS, et al. Breast Cancer Res Treat. 1995;33(2):103-14. doi: 10.1007/BF00682718. Breast Cancer Res Treat. 1995. PMID: 7749138 Review. - Epidermal growth factor-related peptides in the pathogenesis of human breast cancer.
Normanno N, Ciardiello F, Brandt R, Salomon DS. Normanno N, et al. Breast Cancer Res Treat. 1994 Jan;29(1):11-27. doi: 10.1007/BF00666178. Breast Cancer Res Treat. 1994. PMID: 7912564 Review.
Cited by
- Targeted EGFR Nanotherapy in Non-Small Cell Lung Cancer.
Crintea A, Constantin AM, Motofelea AC, Crivii CB, Velescu MA, Coșeriu RL, Ilyés T, Crăciun AM, Silaghi CN. Crintea A, et al. J Funct Biomater. 2023 Sep 9;14(9):466. doi: 10.3390/jfb14090466. J Funct Biomater. 2023. PMID: 37754880 Free PMC article. Review. - Amphiregulin couples IL1RL1+ regulatory T cells and cancer-associated fibroblasts to impede antitumor immunity.
Sun R, Zhao H, Gao DS, Ni A, Li H, Chen L, Lu X, Chen K, Lu B. Sun R, et al. Sci Adv. 2023 Aug 25;9(34):eadd7399. doi: 10.1126/sciadv.add7399. Epub 2023 Aug 23. Sci Adv. 2023. PMID: 37611111 Free PMC article. - The role of amphiregulin in ovarian function and disease.
Fang L, Sun YP, Cheng JC. Fang L, et al. Cell Mol Life Sci. 2023 Feb 7;80(3):60. doi: 10.1007/s00018-023-04709-8. Cell Mol Life Sci. 2023. PMID: 36749397 Free PMC article. Review. - Regulation of the Hippo signaling pathway by deubiquitinating enzymes in cancer.
Mussell A, Frangou C, Zhang J. Mussell A, et al. Genes Dis. 2019 Jun 24;6(4):335-341. doi: 10.1016/j.gendis.2019.06.004. eCollection 2019 Dec. Genes Dis. 2019. PMID: 31832513 Free PMC article. Review. - Transcription-independent Induction of ERBB1 through Hypoxia-inducible Factor 2A Provides Cardioprotection during Ischemia and Reperfusion.
Lee JW, Koeppen M, Seo SW, Bowser JL, Yuan X, Li J, Sibilia M, Ambardekar AV, Zhang X, Eckle T, Yoo SH, Eltzschig HK. Lee JW, et al. Anesthesiology. 2020 Apr;132(4):763-780. doi: 10.1097/ALN.0000000000003037. Anesthesiology. 2020. PMID: 31794514 Free PMC article.
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases
Research Materials
Miscellaneous