Identification of a glycoprotein ligand for E-selectin on mouse myeloid cells - PubMed (original) (raw)

Comparative Study

Identification of a glycoprotein ligand for E-selectin on mouse myeloid cells

A Levinovitz et al. J Cell Biol. 1993 Apr.

Abstract

E-selectin is an inducible endothelial cell adhesion molecule for neutrophils which functions as a Ca(2+)-dependent lectin. Using a recombinant, antibody-like form of mouse E-selectin, we have searched for glycoprotein ligands on mouse neutrophils and the neutrophil progenitor cell line 32D cl 3. We have identified a 150-kD glycoprotein as the only protein which could be affinity-isolated with soluble E-selectin from [35S]methionine/[35S]cysteine-labeled 32D cl 3 cells. Binding of this protein was strictly Ca(2+)-dependent, was blocked by a cell adhesion-blocking mAb against mouse E-selectin, and required the presence of sialic acid on the 150-kD ligand. This glycoprotein was also affinity-isolated from mature neutrophils, in addition to a minor component at 250 kD, but could not be isolated from several other non-myeloid cell lines. The 150-kD glycoprotein was the only protein from 32D cl 3 cells, which was detectable by silver-staining after a one-step affinity-isolation.

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References

1. 1. J Cell Biol. 1991 Feb;112(3):491-9 - PubMed
2. 1. Nature. 1991 Jan 10;349(6305):164-7 - PubMed
3. 1. J Cell Biol. 1991 Jun;113(5):1213-21 - PubMed
4. 1. Proc Natl Acad Sci U S A. 1991 Jul 15;88(14):6224-8 - PubMed
5. 1. J Cell Biol. 1991 Jul;114(2):343-9 - PubMed

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