Structure-specific endonucleolytic cleavage of nucleic acids by eubacterial DNA polymerases - PubMed (original) (raw)
. 1993 May 7;260(5109):778-83.
doi: 10.1126/science.7683443.
Affiliations
- PMID: 7683443
- DOI: 10.1126/science.7683443
Structure-specific endonucleolytic cleavage of nucleic acids by eubacterial DNA polymerases
V Lyamichev et al. Science. 1993.
Abstract
Previously known 5' exonucleases of several eubacterial DNA polymerases have now been shown to be structure-specific endonucleases that cleave single-stranded DNA or RNA at the bifurcated end of a base-paired duplex. Cleavage was not coupled to synthesis, although primers accelerated the rate of cleavage considerably. The enzyme appeared to gain access to the cleavage site by moving from the free end of a 5' extension to the bifurcation of the duplex, where cleavage took place. Single-stranded 5' arms up to 200 nucleotides long were cleaved from such a duplex. Essentially any linear single-stranded nucleic acid can be targeted for specific cleavage by the 5' nuclease of DNA polymerase through hybridization with an oligonucleotide that converts the desired cleavage site into a substrate.
Similar articles
- Trapping DNA polymerases using triplex-forming oligodeoxyribonucleotides.
Samadashwily GM, Mirkin SM. Samadashwily GM, et al. Gene. 1994 Nov 4;149(1):127-36. doi: 10.1016/0378-1119(94)90421-9. Gene. 1994. PMID: 7958976 - Heterogeneity of primer extension products in asymmetric PCR is due both to cleavage by a structure-specific exo/endonuclease activity of DNA polymerases and to premature stops.
Tombline G, Bellizzi D, Sgaramella V. Tombline G, et al. Proc Natl Acad Sci U S A. 1996 Apr 2;93(7):2724-8. doi: 10.1073/pnas.93.7.2724. Proc Natl Acad Sci U S A. 1996. PMID: 8610108 Free PMC article. - Relief of DNA polymerase stop(s) due to severity of secondary structure of single-stranded DNA template during DNA sequencing.
Ranu RS. Ranu RS. Anal Biochem. 1994 Feb 15;217(1):158-61. doi: 10.1006/abio.1994.1099. Anal Biochem. 1994. PMID: 8203733 No abstract available. - Circular oligonucleotides: new concepts in oligonucleotide design.
Kool ET. Kool ET. Annu Rev Biophys Biomol Struct. 1996;25:1-28. doi: 10.1146/annurev.bb.25.060196.000245. Annu Rev Biophys Biomol Struct. 1996. PMID: 8800462 Review. - Boranophosphates as mimics of natural phosphodiesters in DNA.
Summers JS, Shaw BR. Summers JS, et al. Curr Med Chem. 2001 Aug;8(10):1147-55. doi: 10.2174/0929867013372409. Curr Med Chem. 2001. PMID: 11472233 Review.
Cited by
- Generic Reporter Sets for Colorimetric Multiplex dPCR Demonstrated with 6-Plex SNP Quantification Panels.
Neugebauer M, Calabrese S, Müller S, Truong TT, Juelg P, Borst N, Hutzenlaub T, Dazert E, Bubnoff NCCV, von Stetten F, Lehnert M. Neugebauer M, et al. Int J Mol Sci. 2024 Aug 17;25(16):8968. doi: 10.3390/ijms25168968. Int J Mol Sci. 2024. PMID: 39201654 Free PMC article. - Real-time Fluorescent PCR Techniques to Study Microbial-Host Interactions.
Mackay IM, Arden KE, Nitsche A. Mackay IM, et al. Methods Microbiol. 2004;34:255-330. doi: 10.1016/S0580-9517(04)34010-9. Epub 2004 Dec 1. Methods Microbiol. 2004. PMID: 38620210 Free PMC article. Review. - Clamping-mediated incorporation of single-stranded DNA with concomitant DNA synthesis by Taq polymerase involves nick-translation.
Ohtsubo Y, Kawahara S, Nagata Y. Ohtsubo Y, et al. Sci Rep. 2024 Jan 23;14(1):2030. doi: 10.1038/s41598-024-52095-3. Sci Rep. 2024. PMID: 38263346 Free PMC article. - A four-point molecular handover during Okazaki maturation.
Botto MM, Borsellini A, Lamers MH. Botto MM, et al. Nat Struct Mol Biol. 2023 Oct;30(10):1505-1515. doi: 10.1038/s41594-023-01071-y. Epub 2023 Aug 24. Nat Struct Mol Biol. 2023. PMID: 37620586 - Abasic site-peptide cross-links are blocking lesions repaired by AP endonucleases.
Yudkina AV, Bulgakov NA, Kim DV, Baranova SV, Ishchenko AA, Saparbaev MK, Koval VV, Zharkov DO. Yudkina AV, et al. Nucleic Acids Res. 2023 Jul 7;51(12):6321-6336. doi: 10.1093/nar/gkad423. Nucleic Acids Res. 2023. PMID: 37216593 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases