Calcium-dependent heparin-like ligands for L-selectin in nonlymphoid endothelial cells - PubMed (original) (raw)

. 1993 Jul 23;261(5120):480-3.

doi: 10.1126/science.7687382.

Affiliations

• PMID: 7687382
• DOI: 10.1126/science.7687382

Calcium-dependent heparin-like ligands for L-selectin in nonlymphoid endothelial cells

K E Norgard-Sumnicht et al. Science. 1993.

Abstract

L-Selectin is a calcium-dependent mammalian lectin that mediates lymphocyte trafficking by recognizing sialylated ligands on high endothelial venules in lymph nodes. Although L-selectin probably mediates neutrophil extravasation into nonlymphoid tissues, no corresponding ligand has been characterized. Staining of cultured endothelial cells with an L-selectin chimera (LS-Rg) showed an internal pool of ligands. Metabolic labeling with sulfur-35-labeled sulfate revealed heparin lyase-sensitive ligands that bound LS-Rg in a calcium-dependent, sialic acid-independent manner. A fraction of commercial heparin bound to LS-Rg and LS-Rg bound to heparin-agarose, both in a calcium-dependent manner. Thus, L-selectin recognizes endothelial heparin-like chains, which could be physiological ligands mediating leucocyte trafficking.

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