Induction by IL-9 and suppression by IL-3 and IL-4 of the levels of chromosome 14-derived transcripts that encode late-expressed mouse mast cell proteases - PubMed (original) (raw)

. 1993 Oct 15;151(8):4266-73.

Affiliations

PMID: 7691943

Induction by IL-9 and suppression by IL-3 and IL-4 of the levels of chromosome 14-derived transcripts that encode late-expressed mouse mast cell proteases

K K Eklund et al. J Immunol. 1993.

Abstract

Immature, rIL-3-dependent mouse bone marrow-derived mast cells (BMMC) contain high steady-state levels of the mouse mast cell protease (mMCP) 5 transcript but undetectable levels of the mMCP-1, mMCP-2, or mMCP-4 transcripts even though all four of their genes reside at a locus on chromosome 14. These mast cells can be induced by recombinant c-kit ligand (rKL) to obtain high steady-state levels of the mMCP-4 transcript and by rIL-10 to obtain high steady-state levels of the mMCP-1 and mMCP-2 transcripts. rIL-3 and rKL both elicit the differentiation of progenitor cells into immature BMMC and then stimulate their proliferation. We now report that although rIL-9 alone has no effect on BMMC proliferation as assessed by their incorporation of [3H]thymidine, rIL-9 in combination with rKL enhances the long term viability of BMMC. Furthermore, rIL-9 in the presence of rKL stimulates mouse BMMC to undergo a phenotypic change by inducing accumulation of high steady-state levels of the mMCP-1 and mMCP-2 transcripts. In contrast, in BMMC, the presence of rIL-4 suppresses the rIL-9-induced accumulation of the mMCP-1 and mMCP-2 transcripts, the rIL-10-induced accumulation of the mMCP-1 and mMCP-2 transcripts, and the rKL-induced accumulation of the mMCP-4 transcript, but not the rIL-3-induced accumulation of the mMCP-5 transcript. The presence of rIL-3 also suppresses the rIL-9-induced accumulation of the mMCP-1 and mMCP-2 transcripts. Because of their counter-regulatory actions on the steady-state levels of transcripts that encode three late-expressed serine proteases in BALB/cJ mice, rIL-4 and rIL-3 both inhibit the final stages of differentiation and maturation of mast cells. Because rIL-4, unlike rIL-3, is neither an inducer of early-expressed proteases nor alone a proliferative factor for BMMC, the counterregulatory actions of rIL-3 and rIL-4 on differentiation and maturation of these mouse mast cells are independent of their other functions.

PubMed Disclaimer

Cited by

IL-4-BATF signaling directly modulates IL-9 producing mucosal mast cell (MMC9) function in experimental food allergy.
Tomar S, Ganesan V, Sharma A, Zeng C, Waggoner L, Smith A, Kim CH, Licona-Limón P, Reinhardt RL, Flavell RA, Wang YH, Hogan SP. Tomar S, et al. J Allergy Clin Immunol. 2021 Jan;147(1):280-295. doi: 10.1016/j.jaci.2020.08.043. Epub 2020 Oct 15. J Allergy Clin Immunol. 2021. PMID: 33069715 Free PMC article.
Cytokine/anti-cytokine therapy - novel treatments for asthma?
Hansbro PM, Kaiko GE, Foster PS. Hansbro PM, et al. Br J Pharmacol. 2011 May;163(1):81-95. doi: 10.1111/j.1476-5381.2011.01219.x. Br J Pharmacol. 2011. PMID: 21232048 Free PMC article. Review.
Protease phenotype of constitutive connective tissue and of induced mucosal mast cells in mice is regulated by the tissue.
Xing W, Austen KF, Gurish MF, Jones TG. Xing W, et al. Proc Natl Acad Sci U S A. 2011 Aug 23;108(34):14210-5. doi: 10.1073/pnas.1111048108. Epub 2011 Aug 8. Proc Natl Acad Sci U S A. 2011. PMID: 21825171 Free PMC article.
Transforming and differentiation-inducing potential of constitutively activated c-kit mutant genes in the IC-2 murine interleukin-3-dependent mast cell line.
Hashimoto K, Tsujimura T, Moriyama Y, Yamatodani A, Kimura M, Tohya K, Morimoto M, Kitayama H, Kanakura Y, Kitamura Y. Hashimoto K, et al. Am J Pathol. 1996 Jan;148(1):189-200. Am J Pathol. 1996. PMID: 8546206 Free PMC article.

Induction by IL-9 and suppression by IL-3 and IL-4 of the levels of chromosome 14-derived transcripts that encode late-expressed mouse mast cell proteases - PubMed (original) (raw)