The conserved pre-mRNA splicing factor U2AF from Drosophila: requirement for viability - PubMed (original) (raw)
. 1993 Oct 22;262(5133):569-73.
doi: 10.1126/science.7692602.
Affiliations
- PMID: 7692602
- DOI: 10.1126/science.7692602
The conserved pre-mRNA splicing factor U2AF from Drosophila: requirement for viability
R Kanaar et al. Science. 1993.
Abstract
The large subunit of the human pre-messenger RNA splicing factor U2 small nuclear ribonucleoprotein auxiliary factor (hU2AF65) is required for spliceosome assembly in vitro. A complementary DNA clone encoding the large subunit of Drosophila U2AF (dU2AF50) has been isolated. The dU2AF50 protein is closely related to its mammalian counterpart and contains three carboxyl-terminal ribonucleoprotein consensus sequence RNA binding domains and an amino-terminal arginine- and serine-rich (R/S) domain. Recombinant dU2AF50 protein complements mammalian splicing extracts depleted of U2AF activity. Germline transformation of Drosophila with the dU2AF50 complementary DNA rescues a lethal mutation, establishing that the dU2AF50 gene is essential for viability. R/S domains have been found in numerous metazoan splicing factors, but their function is unknown. The mutation in Drosophila U2AF will allow in vivo analysis of a conserved R/S domain-containing general splicing factor.
Similar articles
- Mutations in the small subunit of the Drosophila U2AF splicing factor cause lethality and developmental defects.
Rudner DZ, Kanaar R, Breger KS, Rio DC. Rudner DZ, et al. Proc Natl Acad Sci U S A. 1996 Sep 17;93(19):10333-7. doi: 10.1073/pnas.93.19.10333. Proc Natl Acad Sci U S A. 1996. PMID: 8816800 Free PMC article. - RNA binding activity of heterodimeric splicing factor U2AF: at least one RS domain is required for high-affinity binding.
Rudner DZ, Breger KS, Kanaar R, Adams MD, Rio DC. Rudner DZ, et al. Mol Cell Biol. 1998 Jul;18(7):4004-11. doi: 10.1128/MCB.18.7.4004. Mol Cell Biol. 1998. PMID: 9632785 Free PMC article. - Molecular genetic analysis of the heterodimeric splicing factor U2AF: the RS domain on either the large or small Drosophila subunit is dispensable in vivo.
Rudner DZ, Breger KS, Rio DC. Rudner DZ, et al. Genes Dev. 1998 Apr 1;12(7):1010-21. doi: 10.1101/gad.12.7.1010. Genes Dev. 1998. PMID: 9531538 Free PMC article. - The evolutionary conservation of the splicing apparatus between fission yeast and man.
Wentz-Hunter K, Potashkin J. Wentz-Hunter K, et al. Nucleic Acids Symp Ser. 1995;(33):226-8. Nucleic Acids Symp Ser. 1995. PMID: 8643378 Review. - Looking at mRNA splicing and transport in situ.
Zachar Z, Kramer J, Bingham PM. Zachar Z, et al. Methods Cell Biol. 1994;44:599-611. doi: 10.1016/s0091-679x(08)60934-6. Methods Cell Biol. 1994. PMID: 7707971 Review. No abstract available.
Cited by
- Schistosome W-Linked Genes Inform Temporal Dynamics of Sex Chromosome Evolution and Suggest Candidate for Sex Determination.
Elkrewi M, Moldovan MA, Picard MAL, Vicoso B. Elkrewi M, et al. Mol Biol Evol. 2021 Dec 9;38(12):5345-5358. doi: 10.1093/molbev/msab178. Mol Biol Evol. 2021. PMID: 34146097 Free PMC article. - Deoxynivalenol globally affects the selection of 3' splice sites in human cells by suppressing the splicing factors, U2AF1 and SF1.
Hu Z, Sun Y, Chen J, Zhao Y, Qiao H, Chen R, Wen X, Deng Y, Wen J. Hu Z, et al. RNA Biol. 2020 Apr;17(4):584-595. doi: 10.1080/15476286.2020.1719750. Epub 2020 Feb 6. RNA Biol. 2020. PMID: 31992135 Free PMC article. - RBM-5 modulates U2AF large subunit-dependent alternative splicing in C. elegans.
Zhou C, Gao X, Hu S, Gan W, Xu J, Ma YC, Ma L. Zhou C, et al. RNA Biol. 2018;15(10):1295-1308. doi: 10.1080/15476286.2018.1526540. Epub 2018 Oct 13. RNA Biol. 2018. PMID: 30295127 Free PMC article. - Mud2 functions in transcription by recruiting the Prp19 and TREX complexes to transcribed genes.
Minocha R, Popova V, Kopytova D, Misiak D, Hüttelmaier S, Georgieva S, Sträßer K. Minocha R, et al. Nucleic Acids Res. 2018 Oct 12;46(18):9749-9763. doi: 10.1093/nar/gky640. Nucleic Acids Res. 2018. PMID: 30053068 Free PMC article. - Recognition of the 3' splice site RNA by the U2AF heterodimer involves a dynamic population shift.
Voith von Voithenberg L, Sánchez-Rico C, Kang HS, Madl T, Zanier K, Barth A, Warner LR, Sattler M, Lamb DC. Voith von Voithenberg L, et al. Proc Natl Acad Sci U S A. 2016 Nov 15;113(46):E7169-E7175. doi: 10.1073/pnas.1605873113. Epub 2016 Oct 31. Proc Natl Acad Sci U S A. 2016. PMID: 27799531 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases