A PCR-based sex-determination assay in cattle based on the bovine amelogenin locus - PubMed (original) (raw)
A PCR-based sex-determination assay in cattle based on the bovine amelogenin locus
S Ennis et al. Anim Genet. 1994 Dec.
Abstract
A method for determining the sex of bovine embryos has been established. Primers for a portion of the bovine amelogenin locus (AMX/Y) were used to amplify DNA present in either 0.1 microliter of blood or biopsies taken from 6-7-day-old embryos. The primers amplify a 280 bp band in females and a 280 and 217 bp bands in males. The method is rapid, does not require prior purification of DNA and contains an internal control which detects PCR failure.
Similar articles
- Amelogenin cross-amplification in the family Bovidae and its application for sex determination.
Weikard R, Pitra C, Kühn C. Weikard R, et al. Mol Reprod Dev. 2006 Oct;73(10):1333-7. doi: 10.1002/mrd.20554. Mol Reprod Dev. 2006. PMID: 16865718 - Sex identification of Japanese black bear, Ursus thibetanus japonicus, by PCR based on amelogenin gene.
Yamamoto K, Tsubota T, Komatsu T, Katayama A, Murase T, Kita I, Kudo T. Yamamoto K, et al. J Vet Med Sci. 2002 Jun;64(6):505-8. doi: 10.1292/jvms.64.505. J Vet Med Sci. 2002. PMID: 12130835 - PCR amplification of animal DNA with human X-Y amelogenin primers used in gender determination.
Buel E, Wang G, Schwartz M. Buel E, et al. J Forensic Sci. 1995 Jul;40(4):641-4. J Forensic Sci. 1995. PMID: 7595303 - Sex determination by PCR analysis of the X-Y amelogenin gene.
Akane A. Akane A. Methods Mol Biol. 1998;98:245-9. doi: 10.1385/0-89603-443-7:245. Methods Mol Biol. 1998. PMID: 9664568 No abstract available.
Cited by
- Demographic mechanisms underpinning genetic assimilation of remnant groups of a large carnivore.
Mikle N, Graves TA, Kovach R, Kendall KC, Macleod AC. Mikle N, et al. Proc Biol Sci. 2016 Sep 28;283(1839):20161467. doi: 10.1098/rspb.2016.1467. Proc Biol Sci. 2016. PMID: 27655768 Free PMC article. - Transcriptomic Profiling of Circular RNAs in the Goat Rumen During Fetal and Prepubertal Period.
Zhong T, Wang C, Wang M, Zhan S, Freitas-de-Melo A, Wang L, Cao J, Dai D, Guo J, Li L, Zhang H, Niu L. Zhong T, et al. Front Physiol. 2022 Mar 30;13:858991. doi: 10.3389/fphys.2022.858991. eCollection 2022. Front Physiol. 2022. PMID: 35431995 Free PMC article. - Land tenure shapes black bear density and abundance on a multi-use landscape.
Loosen AE, Morehouse AT, Boyce MS. Loosen AE, et al. Ecol Evol. 2018 Dec 18;9(1):73-89. doi: 10.1002/ece3.4617. eCollection 2019 Jan. Ecol Evol. 2018. PMID: 30680097 Free PMC article. - How does spatial study design influence density estimates from spatial capture-recapture models?
Sollmann R, Gardner B, Belant JL. Sollmann R, et al. PLoS One. 2012;7(4):e34575. doi: 10.1371/journal.pone.0034575. Epub 2012 Apr 23. PLoS One. 2012. PMID: 22539949 Free PMC article. - Characteristics of X- and Y-chromosome specific regions of the amelogenin gene and a PCR-based method for sex identification in red deer (Cervus elaphus).
Gurgul A, Radko A, Słota E. Gurgul A, et al. Mol Biol Rep. 2010 Jul;37(6):2915-8. doi: 10.1007/s11033-009-9852-4. Epub 2009 Oct 7. Mol Biol Rep. 2010. PMID: 19809889
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources