Cloning and sequencing of the bacterioferritin gene of Brucella melitensis 16M strain - PubMed (original) (raw)

Comparative Study

. 1995 Mar 20;361(2-3):238-42.

doi: 10.1016/0014-5793(95)00189-g.

Affiliations

Comparative Study

Cloning and sequencing of the bacterioferritin gene of Brucella melitensis 16M strain

P A Denoel et al. FEBS Lett. 1995.

Abstract

The 40 N-terminal amino acids of the 20 kDa antigen A2 from Brucella melitensis were sequenced and showed important similarities with 4 bacterioferrins. A monoclonal antibody raised against this antigen cross-reacted with Escherichia coli bacterioferritin. Hybridization of two sets of degenerate primers with B. melitensis HindIII-digested genomic DNA identified a 3.8 kb fragment. This fragment was shown to contain a bacterioferritin gene (bfr) encoding a 161-amino acid protein. The sequence of the Brucella bacterioferritin is 69% similar to that of E. coli, and many of the ferroxidase centre and haem-ligation residues are conserved.

PubMed Disclaimer

Publication types

MeSH terms

Substances

LinkOut - more resources