Alteration of lipid composition modulates Fc epsilon RI signaling in RBL-2H3 cells - PubMed (original) (raw)
. 1995 Apr 4;34(13):4376-84.
doi: 10.1021/bi00013a029.
Affiliations
- PMID: 7703251
- DOI: 10.1021/bi00013a029
Alteration of lipid composition modulates Fc epsilon RI signaling in RBL-2H3 cells
E Y Chang et al. Biochemistry. 1995.
Abstract
We have used sonicated liposomes of phosphatidylcholine (PC), sphingomyelin (SM), or a mixture of cholesterol (chol) and PC to investigate the role of cellular lipid composition in Fc epsilon RI-mediated stimulation of RBL-2H3 cells. Overnight treatment with either PC or SM liposomes causes a substantial enhancement of antigen-stimulated degranulation and phospholipase A2 activity, whereas treatment with a PC/chol mixture results in partial inhibition of the antigen-stimulated response. The most consistent change in the cellular lipid composition that results from the PC and SM liposome treatments is an approximate 40% decrease in the chol/phospholipid (PL) ratio. The lipid treatments do not alter degranulation stimulated by AlF4- or by Ca2+ ionophore in the presence or absence of PMA, suggesting that lipid alteration affects a receptor-specific signaling process. The lipid treatments do not appear to alter antigen-stimulated tyrosine phosphorylation or Ca2+ mobilization. Possible involvement of protein kinase C (PKC) activation in the signal-enhancing effect of the PL treatments was investigated by using calphostin C and phorbol-12-myristol-13-acetate (PMA) to inhibit PKC activity and degranulation in RBL-2H3 cells. Both SM and PC treatment restore the antigen-mediated degranulation response that is inhibited by long-term treatment (> or = 16 h) with 100 nM PMA or short-term treatment (10 min) with 5 microM calphostin C. The results indicate that a decreased chol/PL ratio facilitates or enhances the receptor-mediated activation of a PKC-like pathway that plays an important role in Fc epsilon RI-stimulated degranulation.(ABSTRACT TRUNCATED AT 250 WORDS)
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