Secondary structure and orientation of phospholamban reconstituted in supported bilayers from polarized attenuated total reflection FTIR spectroscopy - PubMed (original) (raw)
. 1995 Apr 4;34(13):4448-56.
doi: 10.1021/bi00013a038.
Affiliations
- PMID: 7703259
- DOI: 10.1021/bi00013a038
Secondary structure and orientation of phospholamban reconstituted in supported bilayers from polarized attenuated total reflection FTIR spectroscopy
S A Tatulian et al. Biochemistry. 1995.
Abstract
We have studied the secondary structure of native phospholamban (PLB), a 52-residue integral membrane protein that regulates calcium uptake into the cardiac sarcoplasmic reticulum, as well as its 27-residue carboxy-terminal transmembrane segment (PLB26-52). The relative contents of alpha-helix, beta-strand, and random coil, as well as the spatial orientations of the alpha-helices of these molecules, reconstituted in dimyristoylphosphatidylcholine (DMPC) and 1-palmitoyl-2-oleoylphosphatidylcholine (POPC) bilayer membranes, were determined using polarized attenuated total reflection (ATR) Fourier transform infrared (FTIR) spectroscopy. The major component of the amide I' bands of PLB and PLB26-52 was centered at 1654-1657 cm-1 and was assigned to alpha-helix. The fraction of alpha-helix in native PLB was 64-67% (33-35 residues), and the transmembrane peptide PLB26-52 contained 73-82% alpha-helix (20-22 residues); small fractions of beta- and random structures were also identified. The orientational order parameter (S) of the alpha-helical component of PLB26-52 in DMPC was S = 0.86 +/- 0.09, indicating that the transmembrane helix was oriented approximately perpendicular to the membrane plane. Assuming the transmembrane domain of PLB resembles the peptide PLB26-52, the additional alpha-helical residues in PLB were assigned to the cytoplasmic helix and determined to have an order parameter S = -0.15 +/- 0.30. This may imply that the cytoplasmic helix was tilted from the membrane normal by an angle of 61 +/- 13 degrees or, alternatively, may indicate a wide angular distribution.(ABSTRACT TRUNCATED AT 250 WORDS)
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