Heparin protects Opa+ Neisseria gonorrhoeae from the bactericidal action of normal human serum - PubMed (original) (raw)
Comparative Study
Heparin protects Opa+ Neisseria gonorrhoeae from the bactericidal action of normal human serum
T Chen et al. Infect Immun. 1995 May.
Abstract
The pathobiological significance of lipooligosaccharide (LOS) and outer membrane opacity protein (Opa) changes in gonorrheal disease are poorly understood. We assessed variants of strain MS11mk with different LOS and Opa phenotypes for their liability to killing by normal human sera. LOS differences correlated with strikingly disparate susceptibilities to serum killing; LOSa variants were serum resistant, LOSb variants were serum sensitive, and sialylation of LOSb variants enhanced their survival (as reported previously). Opa phenotype had little influence on the killing of serum-sensitive LOSb cells that were incubated directly in normal human sera, but preincubation of Opa+ LOSb variants in heparin increased their serum resistance whereas Opa- LOSb variants showed no change. Some Opa proteins conferred slightly higher resistance than others, but heparin preincubation increased serum resistance for variants expressing each of seven Opa proteins. These in vitro phenomena may relate to conditions within the male urethra where sulfate-containing proteoglycans are abundant and where antibody and complement may transude from blood plasma. The results suggest that the selective advantage for Opa+ Neisseria gonorrhoeae bacteria observed in vivo may reflect their ability to utilize host cell components to resist killing by host defenses.
Similar articles
- Selection of Opa+ Neisseria gonorrhoeae by limited availability of normal human serum.
Bos MP, Hogan D, Belland RJ. Bos MP, et al. Infect Immun. 1997 Feb;65(2):645-50. doi: 10.1128/iai.65.2.645-650.1997. Infect Immun. 1997. PMID: 9009326 Free PMC article. - Neisseria gonorrhoeae scavenges host sialic acid for Siglec-mediated, complement-independent suppression of neutrophil activation.
Cardenas AJ, Thomas KS, Broden MW, Ferraro NJ, Pires MM, John CM, Jarvis GA, Criss AK. Cardenas AJ, et al. mBio. 2024 May 8;15(5):e0011924. doi: 10.1128/mbio.00119-24. Epub 2024 Apr 9. mBio. 2024. PMID: 38587424 Free PMC article. - The contrasting mechanisms of serum resistance of Neisseria gonorrhoeae and group B Neisseria meningitidis.
Ram S, Mackinnon FG, Gulati S, McQuillen DP, Vogel U, Frosch M, Elkins C, Guttormsen HK, Wetzler LM, Oppermann M, Pangburn MK, Rice PA. Ram S, et al. Mol Immunol. 1999 Sep-Oct;36(13-14):915-28. doi: 10.1016/s0161-5890(99)00114-5. Mol Immunol. 1999. PMID: 10698346 Review. - Neisserial surface variation: how and why?
Swanson J, Belland RJ, Hill SA. Swanson J, et al. Curr Opin Genet Dev. 1992 Oct;2(5):805-11. doi: 10.1016/s0959-437x(05)80143-1. Curr Opin Genet Dev. 1992. PMID: 1360853 Review.
Cited by
- Mechanisms of host manipulation by Neisseria gonorrhoeae.
Walker E, van Niekerk S, Hanning K, Kelton W, Hicks J. Walker E, et al. Front Microbiol. 2023 Feb 3;14:1119834. doi: 10.3389/fmicb.2023.1119834. eCollection 2023. Front Microbiol. 2023. PMID: 36819065 Free PMC article. Review. - Distinct Patterns of Host Adherence by Neisseria gonorrhoeae Isolated from Experimental Gonorrhea.
He Y, Zhang S, Zhang Y, Wu B, Xue Y, Ye C, Li Q, Olivia AN, Tembo JM, Chen H, Cai H, Chen T. He Y, et al. Can J Infect Dis Med Microbiol. 2021 May 13;2021:7865405. doi: 10.1155/2021/7865405. eCollection 2021. Can J Infect Dis Med Microbiol. 2021. PMID: 34093925 Free PMC article. - The surface lipoproteins of gram-negative bacteria: Protectors and foragers in harsh environments.
Cole GB, Bateman TJ, Moraes TF. Cole GB, et al. J Biol Chem. 2021 Jan-Jun;296:100147. doi: 10.1074/jbc.REV120.008745. Epub 2020 Dec 10. J Biol Chem. 2021. PMID: 33277359 Free PMC article. Review. - Role of the Gonococcal Neisserial Heparin Binding Antigen in Microcolony Formation, and Serum Resistance and Adherence to Epithelial Cells.
Semchenko EA, Mubaiwa TD, Day CJ, Seib KL. Semchenko EA, et al. J Infect Dis. 2020 Apr 27;221(10):1612-1622. doi: 10.1093/infdis/jiz628. J Infect Dis. 2020. PMID: 31781772 Free PMC article. - Glycointeractome of Neisseria gonorrhoeae: Identification of Host Glycans Targeted by the Gonococcus To Facilitate Adherence to Cervical and Urethral Epithelial Cells.
Semchenko EA, Everest-Dass AV, Jen FE, Mubaiwa TD, Day CJ, Seib KL. Semchenko EA, et al. mBio. 2019 Jul 9;10(4):e01339-19. doi: 10.1128/mBio.01339-19. mBio. 2019. PMID: 31289181 Free PMC article.
References
- Cell. 1989 Feb 24;56(4):539-47 - PubMed
- J Exp Med. 1988 Dec 1;168(6):2121-9 - PubMed
- Clin Microbiol Rev. 1989 Apr;2 Suppl:S112-7 - PubMed
- Clin Microbiol Rev. 1989 Apr;2 Suppl:S29-34 - PubMed
- Annu Rev Microbiol. 1989;43:121-45 - PubMed
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Medical