D-serine, an endogenous synaptic modulator: localization to astrocytes and glutamate-stimulated release - PubMed (original) (raw)

D-serine, an endogenous synaptic modulator: localization to astrocytes and glutamate-stimulated release

M J Schell et al. Proc Natl Acad Sci U S A. 1995.

Abstract

Using an antibody highly specific for D-serine conjugated to glutaraldehyde, we have localized endogenous D-serine in rat brain. Highest levels of D-serine immunoreactivity occur in the gray matter of the cerebral cortex, hippocampus, anterior olfactory nucleus, olfactory tubercle, and amygdala. Localizations of D-serine immunoreactivity correlate closely with those of D-serine binding to the glycine modulatory site of the N-methyl-D-aspartate (NMDA) receptor as visualized by autoradiography and are inversely correlated to the presence of D-amino acid oxidase. D-Serine is enriched in process-bearing glial cells in neuropil with the morphology of protoplasmic astrocytes. In glial cultures of rat cerebral cortex, D-serine is enriched in type 2 astrocytes. The release of D-serine from these cultures is stimulated by agonists of non-NMDA glutamate receptors, suggesting a mechanism by which astrocyte-derived D-serine could modulate neurotransmission. D-Serine appears to be the endogenous ligand for the glycine site of NMDA receptors.

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References

    1. Nature. 1967 May 13;214(5089):681-3 - PubMed
    1. Brain Res. 1994 Aug 1;652(2):297-303 - PubMed
    1. Comp Biochem Physiol. 1969 Mar;28(3):1345-55 - PubMed
    1. Nature. 1970 Mar 28;225(5239):1258-9 - PubMed
    1. Fed Proc. 1973 Oct;32(10):2039-47 - PubMed

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