Oral immunization with a recombinant bacterial antigen produced in transgenic plants - PubMed (original) (raw)

. 1995 May 5;268(5211):714-6.

doi: 10.1126/science.7732379.

Affiliations

Oral immunization with a recombinant bacterial antigen produced in transgenic plants

T A Haq et al. Science. 1995.

Abstract

The binding subunit of Escherichia coli heat-labile enterotoxin (LT-B) is a highly active oral immunogen. Transgenic tobacco and potato plants were made with the use of genes encoding LT-B or an LT-B fusion protein with a microsomal retention sequence. The plants expressed the foreign peptides, both of which formed oligomers that bound the natural ligand. Mice immunized by gavage produced serum and gut mucosal anti-LT-B immunoglobulins that neutralized the enterotoxin in cell protection assays. Feeding mice fresh transgenic potato tubers also caused oral immunization.

PubMed Disclaimer

Comment in

Publication types

MeSH terms

Substances

LinkOut - more resources