Analysis of monosaccharide composition of mucin oligosaccharide alditols by high-performance anion-exchange chromatography - PubMed (original) (raw)
Comparative Study
. 1995 Jan 20;224(2):538-41.
doi: 10.1006/abio.1995.1084.
Affiliations
- PMID: 7733455
- DOI: 10.1006/abio.1995.1084
Comparative Study
Analysis of monosaccharide composition of mucin oligosaccharide alditols by high-performance anion-exchange chromatography
N G Karlsson et al. Anal Biochem. 1995.
Abstract
A simple one-step method for the analysis of monosaccharides including galactosaminitol after acidic hydrolysis is described. The hydrolyzate was re-N-acetylated and analyzed by high-performance anion-exchange chromatography and the sugars were detected by a pulsed amperometric detector. The method was applied on a mixture of neutral oligosaccharides released from mucin glycopeptides of rat small intestine by alkaline borohydride. Sugars were detected down to the nanomole range and the results were compared with monosaccharide compositional analysis performed by gas chromatography of acetylated alditols.
Similar articles
- High-performance anion exchange-chromatography of neutral milk oligosaccharides and oligosaccharide alditols derived from mucin glycoproteins.
Reddy GP, Bush CA. Reddy GP, et al. Anal Biochem. 1991 Nov 1;198(2):278-84. doi: 10.1016/0003-2697(91)90425-s. Anal Biochem. 1991. PMID: 1799211 - Sulphated mucin oligosaccharides from porcine small intestine analysed by four-sector tandem mass spectrometry.
Karlsson NG, Karlsson H, Hansson GC. Karlsson NG, et al. J Mass Spectrom. 1996 May;31(5):560-72. doi: 10.1002/(SICI)1096-9888(199605)31:5<560::AID-JMS331>3.0.CO;2-0. J Mass Spectrom. 1996. PMID: 8799292 - High-performance anion-exchange chromatography with pulsed amperometric detection for carbohydrate analysis of glycoproteins.
Rohrer JS, Basumallick L, Hurum D. Rohrer JS, et al. Biochemistry (Mosc). 2013 Jul;78(7):697-709. doi: 10.1134/S000629791307002X. Biochemistry (Mosc). 2013. PMID: 24010833 Review. - High-performance liquid chromatography of mono- and oligosaccharides.
Honda S. Honda S. Anal Biochem. 1984 Jul;140(1):1-47. doi: 10.1016/0003-2697(84)90130-1. Anal Biochem. 1984. PMID: 6385768 Review. No abstract available.
Cited by
- Intestinal mucins from cystic fibrosis mice show increased fucosylation due to an induced Fucalpha1-2 glycosyltransferase.
Thomsson KA, Hinojosa-Kurtzberg M, Axelsson KA, Domino SE, Lowe JB, Gendler SJ, Hansson GC. Thomsson KA, et al. Biochem J. 2002 Nov 1;367(Pt 3):609-16. doi: 10.1042/BJ20020371. Biochem J. 2002. PMID: 12164788 Free PMC article. - Mucus glycoproteins from pig gastric mucosa: identification ofdifferent mucin populations from the surface epithelium.
Nordman H, Davies JR, Herrmann A, Karlsson NG, Hansson GC, Carlstedt I. Nordman H, et al. Biochem J. 1997 Sep 15;326 ( Pt 3)(Pt 3):903-10. doi: 10.1042/bj3260903. Biochem J. 1997. PMID: 9307044 Free PMC article. - Multiple-reaction monitoring liquid chromatography mass spectrometry for monosaccharide compositional analysis of glycoproteins.
Hammad LA, Saleh MM, Novotny MV, Mechref Y. Hammad LA, et al. J Am Soc Mass Spectrom. 2009 Jun;20(6):1224-34. doi: 10.1016/j.jasms.2009.02.022. Epub 2009 Feb 24. J Am Soc Mass Spectrom. 2009. PMID: 19318280 - A conotoxin from Conus textile with unusual posttranslational modifications reduces presynaptic Ca2+ influx.
Rigby AC, Lucas-Meunier E, Kalume DE, Czerwiec E, Hambe B, Dahlqvist I, Fossier P, Baux G, Roepstorff P, Baleja JD, Furie BC, Furie B, Stenflo J. Rigby AC, et al. Proc Natl Acad Sci U S A. 1999 May 11;96(10):5758-63. doi: 10.1073/pnas.96.10.5758. Proc Natl Acad Sci U S A. 1999. PMID: 10318957 Free PMC article. - Variant size- and glycoforms of the scavenger receptor cysteine-rich protein gp-340 with differential bacterial aggregation.
Eriksson C, Frängsmyr L, Danielsson Niemi L, Loimaranta V, Holmskov U, Bergman T, Leffler H, Jenkinson HF, Strömberg N. Eriksson C, et al. Glycoconj J. 2007 Apr;24(2-3):131-42. doi: 10.1007/s10719-006-9020-1. Epub 2007 Jan 23. Glycoconj J. 2007. PMID: 17243023