Direct interaction of nucleoporin p62 with mRNA during its export from the nucleus - PubMed (original) (raw)

. 1995 Jan:108 ( Pt 1):257-63.

doi: 10.1242/jcs.108.1.257.

Affiliations

• PMID: 7738103
• DOI: 10.1242/jcs.108.1.257

Direct interaction of nucleoporin p62 with mRNA during its export from the nucleus

C Dargemont et al. J Cell Sci. 1995 Jan.

Abstract

Primary transcripts in eukaryotic cells undergo several processing steps within the nucleus, and resulting mature RNA molecules are selectively exported to the cytoplasm. Nucleo-cytoplasmic mRNA transport is an active process that likely involves RNA-protein interactions. To identify specific RNA-binding proteins, we designed a novel approach, which allows the analysis of interactions between mRNAs and proteins along the transport pathway. The method consists of inducing in vivo a covalent binding between nuclear proteins and microinjected mRNAs. Using such a procedure, we were able to detect a direct interaction between nucleoporin p62 with mRNA during export. The formation of the mRNA-p62 complex was inhibited by wheat-germ agglutinin, an inhibitor of mRNA export. Antibodies directed against p62 caused a substantial reduction in the rate of mRNA export from the nucleus.

PubMed Disclaimer

Similar articles

• Cytoplasmic transport of ribosomal subunits microinjected into the Xenopus laevis oocyte nucleus: a generalized, facilitated process.
  Bataillé N, Helser T, Fried HM. Bataillé N, et al. J Cell Biol. 1990 Oct;111(4):1571-82. doi: 10.1083/jcb.111.4.1571. J Cell Biol. 1990. PMID: 2211825 Free PMC article.
• Export of mRNA from microinjected nuclei of Xenopus laevis oocytes.
  Dargemont C, Kühn LC. Dargemont C, et al. J Cell Biol. 1992 Jul;118(1):1-9. doi: 10.1083/jcb.118.1.1. J Cell Biol. 1992. PMID: 1618896 Free PMC article.
• Nuclear export of mRNA by TAP/NXF1 requires two nucleoporin-binding sites but not p15.
  Braun IC, Herold A, Rode M, Izaurralde E. Braun IC, et al. Mol Cell Biol. 2002 Aug;22(15):5405-18. doi: 10.1128/MCB.22.15.5405-5418.2002. Mol Cell Biol. 2002. PMID: 12101235 Free PMC article.
• Messenger RNA export from the nucleus: a series of molecular wardrobe changes.
  Kelly SM, Corbett AH. Kelly SM, et al. Traffic. 2009 Sep;10(9):1199-208. doi: 10.1111/j.1600-0854.2009.00944.x. Epub 2009 Jun 27. Traffic. 2009. PMID: 19552647 Free PMC article. Review.
• Dynamics and kinetics of nucleo-cytoplasmic mRNA export.
  Mor A, Shav-Tal Y. Mor A, et al. Wiley Interdiscip Rev RNA. 2010 Nov-Dec;1(3):388-401. doi: 10.1002/wrna.41. Epub 2010 Sep 15. Wiley Interdiscip Rev RNA. 2010. PMID: 21956938 Review.

Cited by

• RNA polymerase III transcription in synthetic nuclei assembled in vitro from defined DNA templates.
  Ullman KS, Forbes DJ. Ullman KS, et al. Mol Cell Biol. 1995 Sep;15(9):4873-83. doi: 10.1128/MCB.15.9.4873. Mol Cell Biol. 1995. PMID: 7651406 Free PMC article.
• Structural analysis of the p62 complex, an assembly of O-linked glycoproteins that localizes near the central gated channel of the nuclear pore complex.
  Guan T, Müller S, Klier G, Panté N, Blevitt JM, Haner M, Paschal B, Aebi U, Gerace L. Guan T, et al. Mol Biol Cell. 1995 Nov;6(11):1591-603. doi: 10.1091/mbc.6.11.1591. Mol Biol Cell. 1995. PMID: 8589458 Free PMC article.
• Spelling out the roles of individual nucleoporins in nuclear export of mRNA.
  Tingey M, Li Y, Yu W, Young A, Yang W. Tingey M, et al. Nucleus. 2022 Dec;13(1):170-193. doi: 10.1080/19491034.2022.2076965. Nucleus. 2022. PMID: 35593254 Free PMC article. Review.
• Genetic and structural analysis of the Bacteroides conjugative transposon CTn341.
  Bacic M, Parker AC, Stagg J, Whitley HP, Wells WG, Jacob LA, Smith CJ. Bacic M, et al. J Bacteriol. 2005 Apr;187(8):2858-69. doi: 10.1128/JB.187.8.2858-2869.2005. J Bacteriol. 2005. PMID: 15805532 Free PMC article.
• Targeting and function in mRNA export of nuclear pore complex protein Nup153.
  Bastos R, Lin A, Enarson M, Burke B. Bastos R, et al. J Cell Biol. 1996 Sep;134(5):1141-56. doi: 10.1083/jcb.134.5.1141. J Cell Biol. 1996. PMID: 8794857 Free PMC article.

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Silverchair Information Systems