EGF receptor deletions define a region specifically mediating STAT transcription factor activation - PubMed (original) (raw)

. 1995 May 5;210(1):74-81.

doi: 10.1006/bbrc.1995.1629.

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EGF receptor deletions define a region specifically mediating STAT transcription factor activation

P J Coffer et al. Biochem Biophys Res Commun. 1995.

Abstract

Binding of EGF to its cognate receptor results in receptor-dimerisation, auto-phosphorylation and activation of intracellular signal transduction pathways. Autophosphorylated tyrosine residues in the receptor complex bind to SH2-domain containing signalling molecules and these are then often themselves phosphorylated by the receptor kinase. A critical role for these SH2-binding sites, however, is unclear. We have investigated the stimulation of (SH2-domain containing) STAT transcription factor activity, in comparison with MAP kinase activity, in cell lines expressing EGF receptor deletion mutations. Data indicate that two autophosphorylated tyrosine residues Y1068 and Y1086 are critical for STAT activation in contrast to MAP kinase activation. Significantly, these tyrosine residues conform to a consensus YXXQ binding site and suggest direct binding of STAT-proteins to the EGF receptor.

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