The dishevelled protein is modified by wingless signaling in Drosophila - PubMed (original) (raw)

. 1995 May 1;9(9):1087-97.

doi: 10.1101/gad.9.9.1087.

Affiliations

• PMID: 7744250
• DOI: 10.1101/gad.9.9.1087

Free article

The dishevelled protein is modified by wingless signaling in Drosophila

S Yanagawa et al. Genes Dev. 1995.

Free article

Abstract

Wingless (Wg) is an important signaling molecule in the development of Drosophila, but little is known about its signal transduction pathway. Genetic evidence indicates that another segment polarity gene, dishevelled (dsh) is required for Wg signaling. We have recently developed a cell culture system for Wg protein activity, and using this in vitro system as well as intact Drosophila embryos, we have analyzed biochemical changes in the Dsh protein as a consequence of Wg signaling. We find that Dsh is a phosphoprotein, normally present in the cytoplasm. Wg signaling generates a hyperphosphorylated form of Dsh, which is associated with a membrane fraction. Overexpressed Dsh becomes hyperphosphorylated in the absence of extracellular Wg and increases levels of the Armadillo protein, thereby mimicking the Wg signal. A deletional analysis of Dsh identifies several conserved domains essential for activity, among which is a so-called GLGF/DHR motif. We conclude that dsh, a highly conserved gene, is not merely a permissive factor in Wg signaling but encodes a novel signal transduction molecule, which may function between the Wg receptor and more downstream signaling molecules.

PubMed Disclaimer

Similar articles

• The Drosophila segment polarity gene dishevelled encodes a novel protein required for response to the wingless signal.
  Klingensmith J, Nusse R, Perrimon N. Klingensmith J, et al. Genes Dev. 1994 Jan;8(1):118-30. doi: 10.1101/gad.8.1.118. Genes Dev. 1994. PMID: 8288125
• The wingless signaling pathway is directly involved in Drosophila heart development.
  Park M, Wu X, Golden K, Axelrod JD, Bodmer R. Park M, et al. Dev Biol. 1996 Jul 10;177(1):104-16. doi: 10.1006/dbio.1996.0149. Dev Biol. 1996. PMID: 8660881
• Components of wingless signalling in Drosophila.
  Siegfried E, Wilder EL, Perrimon N. Siegfried E, et al. Nature. 1994 Jan 6;367(6458):76-80. doi: 10.1038/367076a0. Nature. 1994. PMID: 8107779
• Signaling by wingless in Drosophila.
  Klingensmith J, Nusse R. Klingensmith J, et al. Dev Biol. 1994 Dec;166(2):396-414. doi: 10.1006/dbio.1994.1325. Dev Biol. 1994. PMID: 7813765 Review.
• New steps in the Wnt/beta-catenin signal transduction pathway.
  Sakanaka C, Sun TQ, Williams LT. Sakanaka C, et al. Recent Prog Horm Res. 2000;55:225-36. Recent Prog Horm Res. 2000. PMID: 11036939 Review.

Cited by

• Crystallization and preliminary X-ray crystallographic studies of the axin DIX domain.
  Shibata N, Tomimoto Y, Hanamura T, Yamamoto R, Ueda M, Ueda Y, Mizuno N, Ogata H, Komori H, Shomura Y, Kataoka M, Shimizu S, Kondo J, Yamamoto H, Kikuchi A, Higuchi Y. Shibata N, et al. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 Jun 1;63(Pt 6):529-31. doi: 10.1107/S1744309107022579. Epub 2007 May 18. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007. PMID: 17554179 Free PMC article.
• Establishment of the dorsal-ventral axis in Xenopus embryos coincides with the dorsal enrichment of dishevelled that is dependent on cortical rotation.
  Miller JR, Rowning BA, Larabell CA, Yang-Snyder JA, Bates RL, Moon RT. Miller JR, et al. J Cell Biol. 1999 Jul 26;146(2):427-37. doi: 10.1083/jcb.146.2.427. J Cell Biol. 1999. PMID: 10427095 Free PMC article.
• Wnt signaling and mammary tumorigenesis.
  Smalley MJ, Dale TC. Smalley MJ, et al. J Mammary Gland Biol Neoplasia. 2001 Jan;6(1):37-52. doi: 10.1023/a:1009564431268. J Mammary Gland Biol Neoplasia. 2001. PMID: 11467451 Review.
• A screen for identifying genes interacting with armadillo, the Drosophila homolog of beta-catenin.
  Greaves S, Sanson B, White P, Vincent JP. Greaves S, et al. Genetics. 1999 Dec;153(4):1753-66. doi: 10.1093/genetics/153.4.1753. Genetics. 1999. PMID: 10581282 Free PMC article.
• Inhibition of the Wnt signaling pathway by Idax, a novel Dvl-binding protein.
  Hino S, Kishida S, Michiue T, Fukui A, Sakamoto I, Takada S, Asashima M, Kikuchi A. Hino S, et al. Mol Cell Biol. 2001 Jan;21(1):330-42. doi: 10.1128/MCB.21.1.330-342.2001. Mol Cell Biol. 2001. PMID: 11113207 Free PMC article.

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • HighWire

• Other Literature Sources

  • The Lens - Patent Citations Database

• Molecular Biology Databases

  • FlyBase
  • GlyGen glycoinformatics resource