Nuclear export of the human immunodeficiency virus type 1 nucleocytoplasmic shuttle protein Rev is mediated by its activation domain and is blocked by transdominant negative mutants - PubMed (original) (raw)
Nuclear export of the human immunodeficiency virus type 1 nucleocytoplasmic shuttle protein Rev is mediated by its activation domain and is blocked by transdominant negative mutants
A M Szilvay et al. J Virol. 1995 Jun.
Abstract
The human immunodeficiency virus type 1 nucleocytoplasmic shuttle protein Rev moves repeatedly between the cytoplasm, a perinuclear zone, the nucleoli, and nucleoplasmic speckles. In this study, we demonstrated by both indirect immunofluorescence and Western immunoblot analysis that nuclear exit of Rev transdominant negative mutants was defective compared with that of wild-type Rev. The basic and activation domains of Rev signal import and export, respectively, of Rev across the nuclear membrane. In cotransfection experiments, mutants containing mutations of Rev inhibited the nuclear egress of wild-type Rev, thus revealing a novel transdominant negative phenotype.
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References
- Genes Dev. 1994 Jul 1;8(13):1538-47 - PubMed
- Cell. 1994 Dec 2;79(5):767-78 - PubMed
- Cell. 1994 Dec 16;79(6):931-4 - PubMed
- Exp Cell Res. 1959 Apr;17(1):59-75 - PubMed
- Exp Cell Res. 1963;24:SUPPL9:126-43 - PubMed
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