A subpopulation of normal B cells latently infected with Epstein-Barr virus resembles Burkitt lymphoma cells in expressing EBNA-1 but not EBNA-2 or LMP1 - PubMed (original) (raw)

A subpopulation of normal B cells latently infected with Epstein-Barr virus resembles Burkitt lymphoma cells in expressing EBNA-1 but not EBNA-2 or LMP1

F Chen et al. J Virol. 1995 Jun.

Abstract

Using reverse transcription of whole cellular RNA and nested PCR, we have performed experiments mixing different proportions of Epstein-Barr virus (EBV)-carrying and EBV-negative cells. Based on the results, a method that detects viral transcripts for EBNA-1, EBNA-2, LMP1, and LMP2a from less than one positive cell among 10(5) negative cells was developed. With this method we have shown that the EBV DNA positive cells among small, high-density peripheral blood B-lymphocytes of normal healthy persons express EBNA-1-mRNA but not EBNA-2 or LMP1. A similar EBV expression pattern is found in type I Burkitt lymphoma cells. We suggest that the expression pattern in the lymphoma cells reflects the viral strategy in normal resting B cells and meets the requirements of latent persistence.

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References

1. Int J Cancer. 1971 Nov 15;8(3):443-50 - PubMed
1. Proc Natl Acad Sci U S A. 1988 Nov;85(22):8693-6 - PubMed
1. Proc Natl Acad Sci U S A. 1983 Sep;80(18):5665-9 - PubMed
1. Proc Natl Acad Sci U S A. 1984 Nov;81(22):7207-11 - PubMed
1. N Engl J Med. 1986 Apr 3;314(14):874-9 - PubMed

A subpopulation of normal B cells latently infected with Epstein-Barr virus resembles Burkitt lymphoma cells in expressing EBNA-1 but not EBNA-2 or LMP1 - PubMed (original) (raw)