Structural determinants of nuclear receptor assembly on DNA direct repeats - PubMed (original) (raw)

. 1995 May 18;375(6528):203-11.

doi: 10.1038/375203a0.

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Structural determinants of nuclear receptor assembly on DNA direct repeats

F Rastinejad et al. Nature. 1995.

Abstract

Nuclear receptor heterodimers recognize response elements composed of two direct repeats of the consensus sequence 5'-AGGTCA-3' separated by one to five base pairs. The 1.9 A crystal structure of the complex formed by the DNA-binding domains of the 9-cis retinoic acid receptor and thyroid hormone receptor bound to a thyroid-response element shows that the subunits interact through a DNA-supported interface involving the carboxy-terminal extension of the DNA-binding domain of the thyroid hormone receptor. The stereochemistry suggests a mechanism by which heterodimers recognize the inter-half-site spacing between direct repeats.

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