Transcriptional patterns of the mutL-miaA superoperon of Escherichia coli K-12 suggest a model for posttranscriptional regulation - PubMed (original) (raw)
Transcriptional patterns of the mutL-miaA superoperon of Escherichia coli K-12 suggest a model for posttranscriptional regulation
H C Tsui et al. Biochimie. 1994.
Abstract
The complex amiB-mutL-miaA-hfq-hflX-hflK-hflC superoperon of E coli contains important genes for several fundamental cellular processes, including cell-wall hydrolysis (amiB), DNA repair (mutL), tRNA modification (miaA) and proteolysis (hflX-hflK-hflC). We report here the transcriptional pattern and possible posttranscriptional regulation of mutL, miaA and hfq genes of this superoperon. RNase protection analysis of mRNA transcribed from the bacterial chromosome demonstrated that there is co-transcription of mutL and miaA. In addition, two internal promoters, PmiaA and P1hfq were identified and mapped to 201 and 837 nucleotides upstream from the respective translation start sites. PmiaA contains poor matches to the -10 and -35 regions of the sigma-70 RNA polymerase consensus sequences, but it contains multiple potential Fis-binding sites and an upstream AT-rich region with poly(A) sequences. The basic arrangement of Fis-binding sites followed by an AT rich region is shared with promoters for rRNA operons and some of the tRNA and tRNA modification genes. As part of an initial study of mutL and miaA regulation, we measured transcript amounts in isogenic rne, rnc and rne rnc double mutants which are deficient in RNase E, RNase III or both. The amounts of steady state level mutL-miaA cotranscript, PmiaA transcript and P1hfq transcript increased eight-, nine- and three-fold respectively in an rne3071 mutant when compared to the rne+ parent. In contrast, amounts of the three transcripts were the same in an rnc105 mutant and its rnc+ parent. These results indicate that mutL, miaA, and hfq expression could be regulated by multiple mechanisms, including degree of cotranscription from upstream genes, modulation of internal promoter strength, and by RNase E activity. A model is presented for RNase E-mediated posttranscriptional regulation that may coordinate mutL expression with replication and miaA with tRNA amounts under different growth conditions, especially during nutrient upshifts.
Similar articles
- The mutL repair gene of Escherichia coli K-12 forms a superoperon with a gene encoding a new cell-wall amidase.
Tsui HC, Zhao G, Feng G, Leung HC, Winkler ME. Tsui HC, et al. Mol Microbiol. 1994 Jan;11(1):189-202. doi: 10.1111/j.1365-2958.1994.tb00300.x. Mol Microbiol. 1994. PMID: 7511774 - The role of the 'gearbox' in the transcription of essential genes.
Vicente M, Kushner SR, Garrido T, Aldea M. Vicente M, et al. Mol Microbiol. 1991 Sep;5(9):2085-91. doi: 10.1111/j.1365-2958.1991.tb02137.x. Mol Microbiol. 1991. PMID: 1766382 Review. - Regulation of transcription of cell division genes in the Escherichia coli dcw cluster.
Vicente M, Gomez MJ, Ayala JA. Vicente M, et al. Cell Mol Life Sci. 1998 Apr;54(4):317-24. doi: 10.1007/s000180050158. Cell Mol Life Sci. 1998. PMID: 9614967 Free PMC article. Review.
Cited by
- Insights into the complementation potential of the extreme acidophile's orthologue in replacing Escherichia coli hfq gene-particularly in bacterial resistance to environmental stress.
Hu W, Huo X, Bai H, Chen Z, Zhang J, Yang H, Feng S. Hu W, et al. World J Microbiol Biotechnol. 2024 Feb 22;40(4):105. doi: 10.1007/s11274-024-03924-0. World J Microbiol Biotechnol. 2024. PMID: 38386219 - A tRNA modifying enzyme as a tunable regulatory nexus for bacterial stress responses and virulence.
Fleming BA, Blango MG, Rousek AA, Kincannon WM, Tran A, Lewis AJ, Russell CW, Zhou Q, Baird LM, Barber AE, Brannon JR, Beebout CJ, Bandarian V, Hadjifrangiskou M, Howard MT, Mulvey MA. Fleming BA, et al. Nucleic Acids Res. 2022 Jul 22;50(13):7570-7590. doi: 10.1093/nar/gkac116. Nucleic Acids Res. 2022. PMID: 35212379 Free PMC article. - RNA Chaperones Hfq and ProQ Play a Key Role in the Virulence of the Plant Pathogenic Bacterium Dickeya dadantii.
Leonard S, Villard C, Nasser W, Reverchon S, Hommais F. Leonard S, et al. Front Microbiol. 2021 Jun 24;12:687484. doi: 10.3389/fmicb.2021.687484. eCollection 2021. Front Microbiol. 2021. PMID: 34248909 Free PMC article. - Crucial Role of the C-Terminal Domain of Hfq Protein in Genomic Instability.
Parekh VJ, Wien F, Grange W, De Long TA, Arluison V, Sinden RR. Parekh VJ, et al. Microorganisms. 2020 Oct 17;8(10):1598. doi: 10.3390/microorganisms8101598. Microorganisms. 2020. PMID: 33080799 Free PMC article. - TrmL and TusA Are Necessary for rpoS and MiaA Is Required for hfq Expression in Escherichia coli.
Aubee JI, Olu M, Thompson KM. Aubee JI, et al. Biomolecules. 2017 May 4;7(2):39. doi: 10.3390/biom7020039. Biomolecules. 2017. PMID: 28471404 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Molecular Biology Databases
Research Materials