Inactivation of the superior cerebellar peduncle blocks expression but not acquisition of the rabbit's classically conditioned eye-blink response - PubMed (original) (raw)

Comparative Study

Inactivation of the superior cerebellar peduncle blocks expression but not acquisition of the rabbit's classically conditioned eye-blink response

D J Krupa et al. Proc Natl Acad Sci U S A. 1995.

Abstract

The localization of sites of memory formation within the mammalian brain has proven to be a formidable task even for simple forms of learning and memory. Recent studies have demonstrated that reversibly inactivating a localized region of cerebellum, including the dorsal anterior interpositus nucleus, completely prevents acquisition of the conditioned eye-blink response with no effect upon subsequent learning without inactivation. This result indicates that the memory trace for this type of learning is located either (i) within this inactivated region of cerebellum or (ii) within some structure(s) efferent from the cerebellum to which output from the interpositus nucleus ultimately projects. To distinguish between these possibilities, two groups of rabbits were conditioned (by using two conditioning stimuli) while the output fibers of the interpositus (the superior cerebellar peduncle) were reversibly blocked with microinjections of the sodium channel blocker tetrodotoxin. Rabbits performed no conditioned responses during this inactivation training. However, training after inactivation revealed that the rabbits (trained with either conditioned stimulus) had fully learned the response during the previous inactivation training. Cerebellar output, therefore, does not appear to be essential for acquisition of the learned response. This result, coupled with the fact that inactivation of the appropriate region of cerebellum completely prevents learning, provides compelling evidence supporting the hypothesis that the essential memory trace for the classically conditioned eye-blink response is localized within the cerebellum.

PubMed Disclaimer

Cited by

Reversible inactivations of the cerebellum prevent the extinction of conditioned nictitating membrane responses in rabbits.
Ramnani N, Yeo CH. Ramnani N, et al. J Physiol. 1996 Aug 15;495 ( Pt 1)(Pt 1):159-68. doi: 10.1113/jphysiol.1996.sp021581. J Physiol. 1996. PMID: 8866359 Free PMC article.
Multiple components in direction learning in smooth pursuit eye movements of monkeys.
Hall NJ, Yang Y, Lisberger SG. Hall NJ, et al. J Neurophysiol. 2018 Oct 1;120(4):2020-2035. doi: 10.1152/jn.00261.2018. Epub 2018 Aug 1. J Neurophysiol. 2018. PMID: 30067122 Free PMC article.
Differential effects of cerebellar inactivation on eyeblink conditioned excitation and inhibition.
Freeman JH Jr, Halverson HE, Poremba A. Freeman JH Jr, et al. J Neurosci. 2005 Jan 26;25(4):889-95. doi: 10.1523/JNEUROSCI.4534-04.2005. J Neurosci. 2005. PMID: 15673669 Free PMC article.
Learning induces a CDC2-related protein kinase, KKIAMRE.
Gomi H, Sun W, Finch CE, Itohara S, Yoshimi K, Thompson RF. Gomi H, et al. J Neurosci. 1999 Nov 1;19(21):9530-7. doi: 10.1523/JNEUROSCI.19-21-09530.1999. J Neurosci. 1999. PMID: 10531455 Free PMC article.
The role of the cerebellar interpositus nucleus in short and long term memory for trace eyeblink conditioning.
Pakaprot N, Kim S, Thompson RF. Pakaprot N, et al. Behav Neurosci. 2009 Feb;123(1):54-61. doi: 10.1037/a0014263. Behav Neurosci. 2009. PMID: 19170430 Free PMC article.

References

1. J Physiol. 1969 Jun;202(2):437-70 - PubMed
1. Exp Brain Res. 1994;100(3):453-68 - PubMed
1. Brain Res. 1982 Jul 29;244(2):347-50 - PubMed
1. Behav Brain Res. 1983 Dec;10(2-3):393-8 - PubMed
1. Brain Res. 1984 Jan 16;291(1):125-36 - PubMed

Inactivation of the superior cerebellar peduncle blocks expression but not acquisition of the rabbit's classically conditioned eye-blink response - PubMed (original) (raw)