A C-terminally-anchored Golgi protein is inserted into the endoplasmic reticulum and then transported to the Golgi apparatus - PubMed (original) (raw)
Comparative Study
A C-terminally-anchored Golgi protein is inserted into the endoplasmic reticulum and then transported to the Golgi apparatus
A D Linstedt et al. Proc Natl Acad Sci U S A. 1995.
Abstract
Unlike conventional membrane proteins of the secretory pathway, proteins anchored to the cytoplasmic surface of membranes by hydrophobic sequences near their C termini follow a posttranslational, signal recognition particle-independent insertion pathway. Many such C-terminally-anchored proteins have restricted intracellular locations, but it is not known whether these proteins are targeted directly to the membranes in which they will ultimately reside. Here we have analyzed the intracellular sorting of the Golgi protein giantin, which consists of a rod-shaped 376-kDa cytoplasmic domain followed by a hydrophobic C-terminal anchor sequence. Unexpectedly, we find that giantin behaves like a conventional secretory protein in that it inserts into the endoplasmic reticulum (ER) and then is transported to the Golgi. A deletion mutant lacking a portion of the cytoplasmic domain adjacent to the membrane anchor still inserts into the ER but fails to reach the Golgi, even though this mutant has a stable folded structure. These findings suggest that the localization of a C-terminally-anchored Golgi protein involves at least three steps: insertion into the ER membrane, controlled incorporation into transport vesicles, and retention within the Golgi.
Similar articles
- Two endoplasmic reticulum (ER) membrane proteins that facilitate ER-to-Golgi transport of glycosylphosphatidylinositol-anchored proteins.
Barz WP, Walter P. Barz WP, et al. Mol Biol Cell. 1999 Apr;10(4):1043-59. doi: 10.1091/mbc.10.4.1043. Mol Biol Cell. 1999. PMID: 10198056 Free PMC article. - An essential cytoplasmic domain for the Golgi localization of coiled-coil proteins with a COOH-terminal membrane anchor.
Misumi Y, Sohda M, Tashiro A, Sato H, Ikehara Y. Misumi Y, et al. J Biol Chem. 2001 Mar 2;276(9):6867-73. doi: 10.1074/jbc.M010121200. Epub 2000 Dec 11. J Biol Chem. 2001. PMID: 11113150 - Cytoplasmic domain signal sequences that mediate transport of varicella-zoster virus gB from the endoplasmic reticulum to the Golgi.
Heineman TC, Krudwig N, Hall SL. Heineman TC, et al. J Virol. 2000 Oct;74(20):9421-30. doi: 10.1128/jvi.74.20.9421-9430.2000. J Virol. 2000. PMID: 11000211 Free PMC article. - Retention and retrieval in the endoplasmic reticulum and the Golgi apparatus.
Nilsson T, Warren G. Nilsson T, et al. Curr Opin Cell Biol. 1994 Aug;6(4):517-21. doi: 10.1016/0955-0674(94)90070-1. Curr Opin Cell Biol. 1994. PMID: 7986527 Free PMC article. Review. - Retention mechanisms for ER and Golgi membrane proteins.
Gao C, Cai Y, Wang Y, Kang BH, Aniento F, Robinson DG, Jiang L. Gao C, et al. Trends Plant Sci. 2014 Aug;19(8):508-15. doi: 10.1016/j.tplants.2014.04.004. Epub 2014 Apr 30. Trends Plant Sci. 2014. PMID: 24794130 Review.
Cited by
- The C10orf76-PI4KB axis orchestrates CERT-mediated ceramide trafficking to the distal Golgi.
Mizuike A, Sakai S, Katoh K, Yamaji T, Hanada K. Mizuike A, et al. J Cell Biol. 2023 Jul 3;222(7):e202111069. doi: 10.1083/jcb.202111069. Epub 2023 May 17. J Cell Biol. 2023. PMID: 37195633 Free PMC article. - E-Cadherin Modulation and Inter-Cellular Trafficking in Tubular Gastric Adenocarcinoma: A High-Resolution Microscopy Pilot Study.
Liliac IM, Ungureanu BS, Mărgăritescu C, Sacerdoțianu VM, Săftoiu A, Mogoantă L, Moraru E, Pirici D. Liliac IM, et al. Biomedicines. 2022 Feb 1;10(2):349. doi: 10.3390/biomedicines10020349. Biomedicines. 2022. PMID: 35203558 Free PMC article. - Alterations of Golgi Structural Proteins and Glycosylation Defects in Cancer.
Zhang X. Zhang X. Front Cell Dev Biol. 2021 May 12;9:665289. doi: 10.3389/fcell.2021.665289. eCollection 2021. Front Cell Dev Biol. 2021. PMID: 34055798 Free PMC article. Review. - The Golgi Apparatus May Be a Potential Therapeutic Target for Apoptosis-Related Neurological Diseases.
He Q, Liu H, Deng S, Chen X, Li D, Jiang X, Zeng W, Lu W. He Q, et al. Front Cell Dev Biol. 2020 Aug 31;8:830. doi: 10.3389/fcell.2020.00830. eCollection 2020. Front Cell Dev Biol. 2020. PMID: 33015040 Free PMC article. Review. - Post-ER Stress Biogenesis of Golgi Is Governed by Giantin.
Frisbie CP, Lushnikov AY, Krasnoslobodtsev AV, Riethoven JM, Clarke JL, Stepchenkova EI, Petrosyan A. Frisbie CP, et al. Cells. 2019 Dec 13;8(12):1631. doi: 10.3390/cells8121631. Cells. 2019. PMID: 31847122 Free PMC article.
References
- EMBO J. 1994 Feb 1;13(3):562-74 - PubMed
- J Biol Chem. 1992 Apr 15;267(11):7784-93 - PubMed
- Mol Biol Cell. 1993 Jul;4(7):679-93 - PubMed
- Mol Cell Biol. 1994 Apr;14(4):2564-76 - PubMed
- J Biol Chem. 1994 Feb 11;269(6):4035-41 - PubMed
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources