Rethinking cell structure - PubMed (original) (raw)

Review

Rethinking cell structure

S Penman. Proc Natl Acad Sci U S A. 1995.

Abstract

Cell structure, emerging from behind the veil of conventional electron microscopy, appears far more complex than formerly realized. The standard plastic-embedded, ultrathin section can image only what is on the section surface and masks the elaborate networks of the cytoplasm and nucleus. Embedment-free electron microscopy gives clear, high-contrast micrographs of cell structure when combined with removal of obscuring material such as soluble proteins. The resinless ultrathin section is the technique of choice; it is simple and inexpensive, and it uses ordinary electron microscopes. The resulting pictures reveal a world of complex cell structure and function. These images necessarily change our conception of the cytoskeleton, nuclear matrix, mitosis, and the relation of membranes to cytostructure.

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References

1. 1. Proc Natl Acad Sci U S A. 1974 Jun;71(6):2268-72 - PubMed
2. 1. Int Rev Cytol. 1994;150:173-224 - PubMed
3. 1. Science. 1975 Jul 25;189(4199):291-3 - PubMed
4. 1. J Supramol Struct. 1976;5(2):155-83 - PubMed
5. 1. J Cell Biol. 1977 Jun;73(3):616-37 - PubMed

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