Correlation of microscopic phenotype with genotype in a formalin-fixed, paraffin-embedded testicular germ cell tumor with universal DNA amplification, comparative genomic hybridization, and interphase cytogenetics - PubMed (original) (raw)

. 1995 Jun;146(6):1332-40.

Affiliations

PMID: 7778673
PMCID: PMC1870909

Correlation of microscopic phenotype with genotype in a formalin-fixed, paraffin-embedded testicular germ cell tumor with universal DNA amplification, comparative genomic hybridization, and interphase cytogenetics

M R Speicher et al. Am J Pathol. 1995 Jun.

Abstract

We present a strategy for the evaluation of numerical copy number changes of DNA segments within a solid tumor genome that allows the correlation of microscopic phenotype with genotype in formalin-fixed, paraffin-embedded tumor material. Cells from a human testicular germ cell tumor and adjacent tissue areas with normal seminiferous tubules were selected separately from microscopically analyzed histological tissue sections, and DNA was extracted from the selected areas. After universal DNA amplification, the amplification products were subjected to comparative genomic hybridization. The results confirmed balanced chromosome copy numbers for the normal tissue area, although the analysis of the tumor tissue area revealed numerous gains and losses of chromosome segments. The comparative genomic hybridization results were used to select DNA probes for interphase cytogenetics on serial sections. We conclude that this technique allows the screening of selected tissue areas for numerical DNA alterations, thus enabling a direct phenotype-genotype comparison.

PubMed Disclaimer

Cited by

High resolution array-CGH analysis of single cells.
Fiegler H, Geigl JB, Langer S, Rigler D, Porter K, Unger K, Carter NP, Speicher MR. Fiegler H, et al. Nucleic Acids Res. 2007;35(3):e15. doi: 10.1093/nar/gkl1030. Epub 2006 Dec 18. Nucleic Acids Res. 2007. PMID: 17178751 Free PMC article.
Genetic changes in intraductal breast cancer detected by comparative genomic hybridization.
Kuukasjärvi T, Tanner M, Pennanen S, Karhu R, Kallioniemi OP, Isola J. Kuukasjärvi T, et al. Am J Pathol. 1997 Apr;150(4):1465-71. Am J Pathol. 1997. PMID: 9095001 Free PMC article.
Multicolor deconvolution microscopy of thick biological specimens.
Maierhofer C, Gangnus R, Diebold J, Speicher MR. Maierhofer C, et al. Am J Pathol. 2003 Feb;162(2):373-9. doi: 10.1016/S0002-9440(10)63832-1. Am J Pathol. 2003. PMID: 12547696 Free PMC article.
Evidence for an ependymoma tumour suppressor gene in chromosome region 22pter-22q11.2.
Hulsebos TJ, Oskam NT, Bijleveld EH, Westerveld A, Hermsen MA, van den Ouweland AM, Hamel BC, Tijssen CC. Hulsebos TJ, et al. Br J Cancer. 1999 Dec;81(7):1150-4. doi: 10.1038/sj.bjc.6690822. Br J Cancer. 1999. PMID: 10584875 Free PMC article.
Comparative genomic hybridization, loss of heterozygosity, and DNA sequence analysis of single cells.
Klein CA, Schmidt-Kittler O, Schardt JA, Pantel K, Speicher MR, Riethmüller G. Klein CA, et al. Proc Natl Acad Sci U S A. 1999 Apr 13;96(8):4494-9. doi: 10.1073/pnas.96.8.4494. Proc Natl Acad Sci U S A. 1999. PMID: 10200290 Free PMC article.

References

1. Am J Pathol. 1994 Jun;144(6):1203-18 - PubMed
1. Cancer Res. 1994 Apr 1;54(7):1801-6 - PubMed
1. Am J Pathol. 1994 Aug;145(2):356-64 - PubMed
1. Am J Pathol. 1994 Dec;145(6):1253-60 - PubMed
1. Cancer Res. 1995 Mar 1;55(5):1010-3 - PubMed

Correlation of microscopic phenotype with genotype in a formalin-fixed, paraffin-embedded testicular germ cell tumor with universal DNA amplification, comparative genomic hybridization, and interphase cytogenetics - PubMed (original) (raw)