The G-rich auxiliary downstream element has distinct sequence and position requirements and mediates efficient 3' end pre-mRNA processing through a trans-acting factor - PubMed (original) (raw)
The G-rich auxiliary downstream element has distinct sequence and position requirements and mediates efficient 3' end pre-mRNA processing through a trans-acting factor
P S Bagga et al. Nucleic Acids Res. 1995.
Free PMC article
Abstract
A downstream G-rich sequence (GRS), GGGGGAGGUGUGGG, has been previously shown to influence the efficiency of 3' end processing of the SV40 late polyadenylation signal. We have now defined several important parameters for GRS-mediated polyadenylation. The ability of the GRS to influence 3' end processing efficiency was sensitive to individual and multiple point mutations within the element, as well as the position of the element in the downstream region. Competition analysis indicated that the GRS functioned through a titratable trans-acting factor. The GRS-specific DSEF-1 protein was found to be bound to the same population of RNAs as the 64 kDa protein of the general polyadenylation factor CstF, indicating that DSEF-1 is associated with RNA substrates undergoing 3' end processing. Furthermore, an association was obtained between the relative strength of DSEF-1 protein binding to GRS variants and the relative ability of the GRS variants to mediate efficient cleavage in vitro. Finally, mutations in the GRS affected the efficiency of cross-linking of the 64 kDa protein of CstF. These data define a novel class of auxiliary downstream element and suggest an important role for DSEF-1 in 3' end processing.
Similar articles
- DSEF-1 is a member of the hnRNP H family of RNA-binding proteins and stimulates pre-mRNA cleavage and polyadenylation in vitro.
Bagga PS, Arhin GK, Wilusz J. Bagga PS, et al. Nucleic Acids Res. 1998 Dec 1;26(23):5343-50. doi: 10.1093/nar/26.23.5343. Nucleic Acids Res. 1998. PMID: 9826757 Free PMC article. - Auxiliary downstream elements are required for efficient polyadenylation of mammalian pre-mRNAs.
Chen F, Wilusz J. Chen F, et al. Nucleic Acids Res. 1998 Jun 15;26(12):2891-8. doi: 10.1093/nar/26.12.2891. Nucleic Acids Res. 1998. PMID: 9611233 Free PMC article. - An RNA-binding protein specifically interacts with a functionally important domain of the downstream element of the simian virus 40 late polyadenylation signal.
Qian ZW, Wilusz J. Qian ZW, et al. Mol Cell Biol. 1991 Oct;11(10):5312-20. doi: 10.1128/mcb.11.10.5312-5320.1991. Mol Cell Biol. 1991. PMID: 1656229 Free PMC article. - The 64-kilodalton subunit of the CstF polyadenylation factor binds to pre-mRNAs downstream of the cleavage site and influences cleavage site location.
MacDonald CC, Wilusz J, Shenk T. MacDonald CC, et al. Mol Cell Biol. 1994 Oct;14(10):6647-54. doi: 10.1128/mcb.14.10.6647-6654.1994. Mol Cell Biol. 1994. PMID: 7935383 Free PMC article. - Implications of polyadenylation in health and disease.
Curinha A, Oliveira Braz S, Pereira-Castro I, Cruz A, Moreira A. Curinha A, et al. Nucleus. 2014;5(6):508-19. doi: 10.4161/nucl.36360. Epub 2014 Oct 31. Nucleus. 2014. PMID: 25484187 Free PMC article. Review.
Cited by
- The HNRNPF/H RNA binding proteins and disease.
Brownmiller T, Caplen NJ. Brownmiller T, et al. Wiley Interdiscip Rev RNA. 2023 Sep-Oct;14(5):e1788. doi: 10.1002/wrna.1788. Epub 2023 Apr 11. Wiley Interdiscip Rev RNA. 2023. PMID: 37042074 Free PMC article. Review. - Enzymatically amplified linear dbDNATM as a rapid and scalable solution to industrial lentiviral vector manufacturing.
Barreira M, Kerridge C, Jorda S, Olofsson D, Neumann A, Horton H, Smith-Moore S. Barreira M, et al. Gene Ther. 2023 Feb;30(1-2):122-131. doi: 10.1038/s41434-022-00343-4. Epub 2022 May 24. Gene Ther. 2023. PMID: 35606492 Free PMC article. - G-quadruplex occurrence and conservation: more than just a question of guanine-cytosine content.
Vannutelli A, Perreault JP, Ouangraoua A. Vannutelli A, et al. NAR Genom Bioinform. 2022 Mar 4;4(1):lqac010. doi: 10.1093/nargab/lqac010. eCollection 2022 Mar. NAR Genom Bioinform. 2022. PMID: 35261973 Free PMC article. - Emerging Roles of RNA 3'-end Cleavage and Polyadenylation in Pathogenesis, Diagnosis and Therapy of Human Disorders.
Nourse J, Spada S, Danckwardt S. Nourse J, et al. Biomolecules. 2020 Jun 17;10(6):915. doi: 10.3390/biom10060915. Biomolecules. 2020. PMID: 32560344 Free PMC article. Review. - G-quadruplexes offer a conserved structural motif for NONO recruitment to NEAT1 architectural lncRNA.
Simko EAJ, Liu H, Zhang T, Velasquez A, Teli S, Haeusler AR, Wang J. Simko EAJ, et al. Nucleic Acids Res. 2020 Jul 27;48(13):7421-7438. doi: 10.1093/nar/gkaa475. Nucleic Acids Res. 2020. PMID: 32496517 Free PMC article.
References
- Nucleic Acids Res. 1983 Mar 11;11(5):1475-89 - PubMed
- Mol Cell Biol. 1994 Oct;14(10):6647-54 - PubMed
- Mol Cell Biol. 1985 Oct;5(10):2713-9 - PubMed
- Cell. 1987 May 8;49(3):399-406 - PubMed
- Cell. 1988 Jan 29;52(2):221-8 - PubMed
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources