Interaction of Mre11 and Rad50: two proteins required for DNA repair and meiosis-specific double-strand break formation in Saccharomyces cerevisiae - PubMed (original) (raw)

Interaction of Mre11 and Rad50: two proteins required for DNA repair and meiosis-specific double-strand break formation in Saccharomyces cerevisiae

K Johzuka et al. Genetics. 1995 Apr.

Abstract

A temperature-sensitive mre11-1 mutation of Saccharomyces cerevisiae causes defects in meiotic recombination and DNA repair during vegetative growth at a restrictive temperature. We cloned the MRE11 gene and found that it encodes a 643-amino acid protein with a highly acidic region containing a heptad repeat of Asp at its C-terminus and is located downstream of YMR44 near the RNA1 locus on the right arm of chromosome XIII. Transcripts of the MRE11 gene increased transiently and showed the same kinetics as that of the RAD50 gene during meiosis. In a mre11 disruption mutant (mre11::hisG), meiosis-specific double-strand break (DSB) formation is abolished. A comparison of the properties of mre11::hisG and a rad50 deletion mutant (rad50 delta) indicated that both mutants exhibited similar phenotypes in both meiosis and mitosis. Characterization of two double mutants, mre11::hisG rad50 delta and mre11::hisG rad50S, showed that MRE11 and RAD50 belong to the same epistasis group with respect to meiotic DSB formation and mitotic DNA repair. Using a two-hybrid system, we found that Mre11 interacts with Rad50 and itself in vivo. These results suggest that Mre11 and Rad50 proteins work in a complex in DSB formation and DNA repair during vegetative growth.

PubMed Disclaimer

Similar articles

• Functions of the yeast meiotic recombination genes, MRE11 and MRE2.
  Ogawa H, Johzuka K, Nakagawa T, Leem SH, Hagihara AH. Ogawa H, et al. Adv Biophys. 1995;31:67-76. doi: 10.1016/0065-227x(95)99383-z. Adv Biophys. 1995. PMID: 7625279 Review.
• Formation of the yeast Mre11-Rad50-Xrs2 complex is correlated with DNA repair and telomere maintenance.
  Chamankhah M, Xiao W. Chamankhah M, et al. Nucleic Acids Res. 1999 May 15;27(10):2072-9. doi: 10.1093/nar/27.10.2072. Nucleic Acids Res. 1999. PMID: 10219078 Free PMC article.
• mre11S--a yeast mutation that blocks double-strand-break processing and permits nonhomologous synapsis in meiosis.
  Nairz K, Klein F. Nairz K, et al. Genes Dev. 1997 Sep 1;11(17):2272-90. doi: 10.1101/gad.11.17.2272. Genes Dev. 1997. PMID: 9303542 Free PMC article.
• Distinct roles of two separable in vitro activities of yeast Mre11 in mitotic and meiotic recombination.
  Furuse M, Nagase Y, Tsubouchi H, Murakami-Murofushi K, Shibata T, Ohta K. Furuse M, et al. EMBO J. 1998 Nov 2;17(21):6412-25. doi: 10.1093/emboj/17.21.6412. EMBO J. 1998. PMID: 9799249 Free PMC article.
• The multiple roles of the Mre11 complex for meiotic recombination.
  Borde V. Borde V. Chromosome Res. 2007;15(5):551-63. doi: 10.1007/s10577-007-1147-9. Chromosome Res. 2007. PMID: 17674145 Review.

Cited by

• Physical interaction with Spo11 mediates the localisation of Mre11 to chromatin in meiosis and promotes its nuclease activity.
  Aithal R, Nangalia K, Spirek M, Chen D, Klein F, Krejci L. Aithal R, et al. Nucleic Acids Res. 2024 May 8;52(8):4328-4343. doi: 10.1093/nar/gkae111. Nucleic Acids Res. 2024. PMID: 38407383 Free PMC article.
• Divergence and conservation of the meiotic recombination machinery.
  Arter M, Keeney S. Arter M, et al. Nat Rev Genet. 2024 May;25(5):309-325. doi: 10.1038/s41576-023-00669-8. Epub 2023 Nov 30. Nat Rev Genet. 2024. PMID: 38036793 Review.
• The Msh5 complex shows homeostatic localization in response to DNA double-strand breaks in yeast meiosis.
  Shinohara M, Shinohara A. Shinohara M, et al. Front Cell Dev Biol. 2023 May 18;11:1170689. doi: 10.3389/fcell.2023.1170689. eCollection 2023. Front Cell Dev Biol. 2023. PMID: 37274743 Free PMC article.
• Unraveling the Molecular Impact of Sperm DNA Damage on Human Reproduction.
  Finelli R, Moreira BP, Alves MG, Agarwal A. Finelli R, et al. Adv Exp Med Biol. 2022;1358:77-113. doi: 10.1007/978-3-030-89340-8_5. Adv Exp Med Biol. 2022. PMID: 35641867
• Novel mechanistic insights into the role of Mer2 as the keystone of meiotic DNA break formation.
  Rousová D, Nivsarkar V, Altmannova V, Raina VB, Funk SK, Liedtke D, Janning P, Müller F, Reichle H, Vader G, Weir JR. Rousová D, et al. Elife. 2021 Dec 24;10:e72330. doi: 10.7554/eLife.72330. Elife. 2021. PMID: 34951404 Free PMC article.

References

1. 1. Genetics. 1990 Dec;126(4):813-22 - PubMed
2. 1. Proc Natl Acad Sci U S A. 1974 Aug;71(8):3172-6 - PubMed
3. 1. Genetics. 1980 Nov;96(3):567-88 - PubMed
4. 1. Nature. 1989 Jul 20;340(6230):245-6 - PubMed
5. 1. Genetics. 1992 Nov;132(3):651-64 - PubMed

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Europe PubMed Central
  • PubMed Central
  • Silverchair Information Systems

• Other Literature Sources

  • The Lens - Patent Citations

• Molecular Biology Databases

  • BioCyc
  • Gene Ontology
  • Saccharomyces Genome Database

• Research Materials

  • NCI CPTC Antibody Characterization Program

• Miscellaneous

  • NCI CPTAC Assay Portal