Sphingosine-1-phosphate: a platelet-activating sphingolipid released from agonist-stimulated human platelets - PubMed (original) (raw)
. 1995 Jul 1;86(1):193-202.
Affiliations
- PMID: 7795224
Free article
Sphingosine-1-phosphate: a platelet-activating sphingolipid released from agonist-stimulated human platelets
Y Yatomi et al. Blood. 1995.
Free article
Abstract
Sphingosine-1-phosphate (Sph-1-P) is the initial product of catabolism of sphingosine by sphingosine kinase and is cleaved by Sph-1-P lyase to a fatty aldehyde and ethanolamine phosphate. This phosphorylated sphingoid base is not only an intermediary catabolite, but also a bioactive lipid with important functions, including stimulation of cell proliferation in Swiss 3T3 fibroblasts and inhibition of tumor cell motility. In the present study, we examined functional roles of Sph-1-P in human platelets. Sph-1-P induced platelet shape change and aggregation reactions, although it failed to elicit secretion. Sphingosine, ceramide, sphingomyelin, and N,N-dimethylsphingosine did not mimic the positive effects of Sph-1-P on platelets. Subthreshold concentrations of Sph-1-P and weak platelet agonists such as adenosine diphosphate (ADP) and epinephrine synergistically elicited aggregation, which may be important for efficient amplification of platelet activation. Sph-1-P induced intracellular Ca2+ mobilization and the dose-response for Ca2+ release correlated closely with the concentration required for induction of shape change. On addition of [3H]sphingosine to intact platelets, the label was rapidly converted to Sph-1-P, and subsequently to ceramide and sphingomyelin. Interestingly, the Sph-1-P formed was specifically released into medium on stimulation of platelets with physiologic agonists. The amount of Sph-1-P in platelets, as measured by its conversion into radiolabeled N-acetyl-Sph-1-P, was 1.4 nmol/10(9) cells and was about four times higher than the mass of Sph present. When compared by mole percent Sph-1-P/phospholipid, the value for platelets is over 10 times higher than that for neutrophils. Our results suggest that Sph-1-P, rapidly converted from sphingosine, abundantly stored in platelets, and released on the cell activation, may play a physiologic role in thrombosis, hemostasis, and the natural wound-healing processes.
Similar articles
- N,N-dimethylsphingosine inhibition of sphingosine kinase and sphingosine 1-phosphate activity in human platelets.
Yatomi Y, Ruan F, Megidish T, Toyokuni T, Hakomori S, Igarashi Y. Yatomi Y, et al. Biochemistry. 1996 Jan 16;35(2):626-33. doi: 10.1021/bi9515533. Biochemistry. 1996. PMID: 8555236 - Quantification of sphingosine derivatives in human platelets: inducible formation of free sphingosine.
Hisano N, Yatomi Y, Fujino MA, Igarashi Y, Kume S, Ozaki Y. Hisano N, et al. J Biochem. 1998 Feb;123(2):263-8. doi: 10.1093/oxfordjournals.jbchem.a021931. J Biochem. 1998. PMID: 9538201 - Sphingosine 1-phosphate formation and intracellular Ca2+ mobilization in human platelets: evaluation with sphingosine kinase inhibitors.
Yang L, Yatomi Y, Satoh K, Igarashi Y, Ozaki Y. Yang L, et al. J Biochem. 1999 Jul;126(1):84-9. doi: 10.1093/oxfordjournals.jbchem.a022440. J Biochem. 1999. PMID: 10393324 - Sphingosine 1-phosphate: synthesis and release.
Yatomi Y, Ozaki Y, Ohmori T, Igarashi Y. Yatomi Y, et al. Prostaglandins Other Lipid Mediat. 2001 Apr;64(1-4):107-22. doi: 10.1016/s0090-6980(01)00103-4. Prostaglandins Other Lipid Mediat. 2001. PMID: 11324700 Review.
Cited by
- S1P and the birth of platelets.
Hla T, Galvani S, Rafii S, Nachman R. Hla T, et al. J Exp Med. 2012 Nov 19;209(12):2137-40. doi: 10.1084/jem.20122284. J Exp Med. 2012. PMID: 23166370 Free PMC article. Review. - Vascular and Immunobiology of the Circulatory Sphingosine 1-Phosphate Gradient.
Yanagida K, Hla T. Yanagida K, et al. Annu Rev Physiol. 2017 Feb 10;79:67-91. doi: 10.1146/annurev-physiol-021014-071635. Epub 2016 Oct 21. Annu Rev Physiol. 2017. PMID: 27813829 Free PMC article. Review. - Lysophospholipids in laboratory medicine.
Yatomi Y, Kurano M, Ikeda H, Igarashi K, Kano K, Aoki J. Yatomi Y, et al. Proc Jpn Acad Ser B Phys Biol Sci. 2018;94(10):373-389. doi: 10.2183/pjab.94.025. Proc Jpn Acad Ser B Phys Biol Sci. 2018. PMID: 30541965 Free PMC article. Review. - Emerging roles of sphingosylphosphorylcholine in modulating cardiovascular functions and diseases.
Ge D, Yue HW, Liu HH, Zhao J. Ge D, et al. Acta Pharmacol Sin. 2018 Dec;39(12):1830-1836. doi: 10.1038/s41401-018-0036-4. Epub 2018 Jul 26. Acta Pharmacol Sin. 2018. PMID: 30050085 Free PMC article. Review. - Recent advances in the biochemistry of sphingolipidoses.
Kolter T, Sandhoff K. Kolter T, et al. Brain Pathol. 1998 Jan;8(1):79-100. doi: 10.1111/j.1750-3639.1998.tb00138.x. Brain Pathol. 1998. PMID: 9458169 Free PMC article. Review.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Miscellaneous