Monocyte expression of the human prointerleukin 1 beta gene (IL1B) is dependent on promoter sequences which bind the hematopoietic transcription factor Spi-1/PU.1 - PubMed (original) (raw)
Comparative Study
Monocyte expression of the human prointerleukin 1 beta gene (IL1B) is dependent on promoter sequences which bind the hematopoietic transcription factor Spi-1/PU.1
Y Kominato et al. Mol Cell Biol. 1995 Jan.
Abstract
Interleukin-1 beta (IL-1 beta) is produced primarily by stimulated monocytes, suggesting that the IL1B gene, which codes for this protein, depends upon at least one cell-type-specific factor. Our previous characterization of the IL1B promoter indicated that the region between -131 and +12 is sufficient to direct cell-type-specific expression of a reporter gene (F. Shirakawa, K. Saito, C.A. Bonagura, D.L. Galson, M.J. Fenton, A.C. Webb, and P. E. Auron, Mol. Cell. Biol. 13:1332-1344, 1993). We now show that a sequence located between positions -50 and -39 of the IL1B promoter binds the tissue-restricted Ets domain transcription factor Spi-1/PU.1 (Spi-1). Mutation of this site abrogates binding of this factor and reduces the ability of the IL1B promoter to function in macrophages. A second Spi-1 binding site located between positions -115 and -97 also is required for maximal IL1B promoter activity in the presence of the proximal Spi-1 binding site. In addition, an activation domain-deficient Spi-1 expression vector acts as a dominant-negative inhibitor of reporter gene expression in a monocyte cell line. Finally, the IL1B promoter, which is inactive in Spi-1-deficient HeLa cells, is activated in these cells by cotransfection with a Spi-1 expression vector. Thus, the cell-type-specific expression of the IL1B promoter appears to be dependent on the binding of Spi-1.
Similar articles
- Human T-cell leukemia virus type I Tax transactivates the promoter of human prointerleukin-1beta gene through association with two transcription factors, nuclear factor-interleukin-6 and Spi-1.
Tsukada J, Misago M, Serino Y, Ogawa R, Murakami S, Nakanishi M, Tonai S, Kominato Y, Morimoto I, Auron PE, Eto S. Tsukada J, et al. Blood. 1997 Oct 15;90(8):3142-53. Blood. 1997. PMID: 9376596 - Autocrine induction of the human pro-IL-1beta gene promoter by IL-1beta in monocytes.
Toda Y, Tsukada J, Misago M, Kominato Y, Auron PE, Tanaka Y. Toda Y, et al. J Immunol. 2002 Feb 15;168(4):1984-91. doi: 10.4049/jimmunol.168.4.1984. J Immunol. 2002. PMID: 11823535 - NF-IL6 (C/EBPbeta ) vigorously activates il1b gene expression via a Spi-1 (PU.1) protein-protein tether.
Yang Z, Wara-Aswapati N, Chen C, Tsukada J, Auron PE. Yang Z, et al. J Biol Chem. 2000 Jul 14;275(28):21272-7. doi: 10.1074/jbc.M000145200. J Biol Chem. 2000. PMID: 10801783 - PU.1 (Spi-1) autoregulates its expression in myeloid cells.
Chen H, Ray-Gallet D, Zhang P, Hetherington CJ, Gonzalez DA, Zhang DE, Moreau-Gachelin F, Tenen DG. Chen H, et al. Oncogene. 1995 Oct 19;11(8):1549-60. Oncogene. 1995. PMID: 7478579 - Review: transcriptional and post-transcriptional regulation of interleukin 1 gene expression.
Fenton MJ. Fenton MJ. Int J Immunopharmacol. 1992 Apr;14(3):401-11. doi: 10.1016/0192-0561(92)90170-p. Int J Immunopharmacol. 1992. PMID: 1618594 Review.
Cited by
- Glibenclamide-Loaded Nanoparticles Reduce NLRP3 Inflammasome Activation and Modulate miR-223-3p/miR-7-1-5p Expression in THP-1 Cells.
Mancuso R, Citterio LA, Agostini S, Marventano I, La Rosa F, Re F, Seneci P, Saresella M, Clerici M. Mancuso R, et al. Pharmaceuticals (Basel). 2023 Nov 10;16(11):1590. doi: 10.3390/ph16111590. Pharmaceuticals (Basel). 2023. PMID: 38004455 Free PMC article. - Platelet status in cancer cachexia progression in ApcMin/+ mice.
Cunningham P, Unger CA, Patton EA, Aiken A, Browne A, James E, Aladhami AK, Hope Rd MC, VanderVeen BN, Cardaci TD, Murphy EA, Enos RT, Velázquez KT. Cunningham P, et al. Front Immunol. 2023 Aug 28;14:1253587. doi: 10.3389/fimmu.2023.1253587. eCollection 2023. Front Immunol. 2023. PMID: 37701438 Free PMC article. - Using a Network-Based Analysis Approach to Investigate the Involvement of S. aureus in the Pathogenesis of Granulomatosis with Polyangiitis.
Rowland G, Kronbichler A, Smith R, Jayne D, van der Graaf PH, Chelliah V. Rowland G, et al. Int J Mol Sci. 2023 Jan 17;24(3):1822. doi: 10.3390/ijms24031822. Int J Mol Sci. 2023. PMID: 36768148 Free PMC article. - scATACpipe: A nextflow pipeline for comprehensive and reproducible analyses of single cell ATAC-seq data.
Hu K, Liu H, Lawson ND, Zhu LJ. Hu K, et al. Front Cell Dev Biol. 2022 Sep 27;10:981859. doi: 10.3389/fcell.2022.981859. eCollection 2022. Front Cell Dev Biol. 2022. PMID: 36238687 Free PMC article. - Carfilzomib modulates tumor microenvironment to potentiate immune checkpoint therapy for cancer.
Zhou Q, Liang J, Yang T, Liu J, Li B, Li Y, Fan Z, Wang W, Chen W, Yuan S, Xu M, Xu Q, Luan Z, Xia Z, Zhou P, Huang Y, Chen L. Zhou Q, et al. EMBO Mol Med. 2022 Jan 11;14(1):e14502. doi: 10.15252/emmm.202114502. Epub 2021 Dec 13. EMBO Mol Med. 2022. PMID: 34898004 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources