Cloning and characterization of the Drosophila melanogaster CDK5 homolog - PubMed (original) (raw)

Comparative Study

. 1994 Dec 19;356(2-3):317-21.

doi: 10.1016/0014-5793(94)01298-9.

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Comparative Study

Cloning and characterization of the Drosophila melanogaster CDK5 homolog

M R Hellmich et al. FEBS Lett. 1994.

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Abstract

The D. melanogaster homolog of mammalian CDK5 has been cloned and its chromosomal location determined. The gene for Cdk5 consists of 4 exons separated by 3 short introns ranging in size from 61-160 bp. Northern blot analysis revealed a single mRNA of approximately 1.6 kb that is expressed at highest levels in the adult fly. The putative amino acid sequence for Drosophila Cdk5 predicts a protein with a mass of approximately 32 kDa that is 77% identical to its mammalian counter-parts. Drosophila Cdk5 gene is located in polytene chromosomal region 52BC of the right arm of chromosome 2. This study provides the framework for a molecular genetic analysis of CDK5 function.

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